Lanostane triterpenoids from the fruiting bodies of Ganoderma hainanense and their cytotoxic activity

Three undescribed lanostane triterpenoids, 24E-en-11-oxo-ganoderiol D (1), 11β-hydroxy-ganoderiol D (2), and 11β-hydroxy-lucidone H (3) were isolated from the 80% EtOH extract of the fruiting bodies of Ganoderma hainanense. Structural elucidation of all the compounds were performed by spectral methods such as 1 D and 2 D (¹H-¹H COSY, HMQC, and HMBC) NMR spectroscopy. All the triterpenoids were in vitro evaluated for their cytotoxic activities against six mammary adenocarcinoma cell lines (MCF7, MDA-MB-231, SK-BR-3, BT-20, HCC38, and AU565). As a result, compound 3 exhibited significant cytotoxic activities against all tested cell lines with IC₅₀ values less than 20 μM.