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Updated: Sep 6, 2025
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Fluorescence Imaging with One-nanometer Accuracy FIONA
Published on: September 26, 2014
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An alternative to MINFLUX that enables nanometer resolution in a confocal microscope
Luciano A Masullo1,2, Alan M Szalai1, Lucía F Lopez1
1Centro de Investigaciones en Bionanociencias (CIBION), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2390, C1425FQD Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina.
Light, Science & Applications
|June 30, 2022
Summary
Researchers developed RASTMIN, a new single-molecule localization method. This technique achieves nanoscale precision and is easily adaptable to standard confocal microscopes, simplifying advanced imaging.
Area of Science:
- Nanoscale science
- Biophysics
- Physical chemistry
Background:
- Precise localization of single fluorescent emitters is crucial for nanoscale measurements.
- Techniques like single-molecule tracking and super-resolution imaging rely on accurate emitter localization.
- Existing advanced methods like MINFLUX offer high resolution but are technically complex.
Purpose of the Study:
- To introduce RASTMIN, a novel, simplified single-molecule localization method.
- To enable high-precision nanoscale localization using accessible instrumentation.
- To demonstrate RASTMIN's utility in super-resolution imaging applications.
Main Methods:
- Raster scanning a light pattern with a minimum intensity.
- Implementation on a modified standard confocal microscope.
- Localization of single fluorophores and imaging of DNA origami structures.
Main Results:
- Achieved ~1-2 nm localization precision with common fluorophores.
- Demonstrated ease of implementation on standard confocal microscopy setups.
- Successfully applied RASTMIN for single-molecule localization and super-resolution imaging.
Conclusions:
- RASTMIN offers a user-friendly and effective alternative for nanoscale localization.
- The method significantly lowers the barrier to entry for advanced single-molecule imaging.
- RASTMIN facilitates nanoscale physicochemical and biophysical measurements with high precision.
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