Nitric oxide detection using catalytic properties of CuCo-PTC metal organic framework

As a vital gaseous signal molecule involved in various physiological and pathological processes, nitric oxide (NO) has attracted extensive attention in the last few decades. In this work, a copper and cobalt element-doped, biphenyl-(3, 4', 5)-tricarboxylic acid (H₃PTC)-synthesized metal organic framework (CuCo-PTC MOF) composite with catalytic ability was synthesized by solvothermal method. The material can catalyse the oxidation of o-phenylenediamine (OPD) groups by hydrogen peroxide (H₂O₂) to form fluorophores (OPDox) with yellow fluorescence emission and greatly improves its reaction rate. In the presence of NO, OPD will react with NO to produce N-(2-hydrazinophenyl) methylamine, and the group will not react with H₂O₂. Therefore, the concentration of NO can be measured indirectly by comparing the changes of fluorescence intensity in the presence and absence of NO. As the concentration of NO changes, the change of solution colour (from bright yellow to colourless) can also be observed under a 365-nm UV lamp. Furthermore, the method represents high selectivity for NO and shows a fast (within 5 min) and specific fluorescence response toward NO with a linear range from 0.25 to 2.0 μM; the strategy has a limit of detection (LOD) of  0.15 μM. More importantly, the probe was successfully used to detect NO in cell lysate. The recovery was between 98.5 and 103.6%, and the relative standard deviation was between 0.4 and 1.8%. The endogenous NO in cells was successfully detected under the stimulation of L-arginine, which proved the possibility of the probe in real-time and rapid sensing in actual samples and cells. The results indicate that this sensing strategy has the potential to detect NO in the internal environment. Schematic of fluorescence detection of NO.