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Time-resolved absolute radius estimation of vibrating contrast microbubbles using an acoustical camera.

Sander Spiekhout1, Jason Voorneveld1, Benjamin van Elburg2

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The Journal of the Acoustical Society of America
|July 1, 2022
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Summary
This summary is machine-generated.

This study enhances the acoustical camera (AC) to measure absolute microbubble oscillations, enabling precise sizing of ultrasound contrast agents. The extended AC provides full radial dynamics for hundreds of microbubbles hourly.

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Area of Science:

  • Biomedical Engineering
  • Acoustics
  • Materials Science

Background:

  • Ultrasound (US) contrast agents are microbubbles (1-10 μm) crucial for medical imaging.
  • Current methods like acoustical cameras (AC) measure relative microbubble oscillations.
  • Absolute oscillation measurement is needed for precise characterization.

Purpose of the Study:

  • To extend the capabilities of the acoustical camera (AC) to measure absolute microbubble oscillations.
  • To develop a method for determining the absolute radius (R0) of microbubbles.
  • To validate the extended AC's sizing accuracy against established methods.

Main Methods:

  • Insonifying microbubbles with high (25 MHz) and low (1-2.5 MHz) frequency ultrasound waves.
  • Analyzing both amplitude modulation (AM) and phase modulation (PM) of the microbubble response.
  • Utilizing the ratio of PM to AM to calculate the absolute microbubble radius (R0).

Main Results:

  • The extended AC successfully measures absolute microbubble oscillations.
  • Phase modulation (PM) from microbubble wall displacement was identified as a key indicator.
  • Sizing accuracy was validated by matching AC-derived size distributions to Coulter counter data for monodisperse populations (2.1 and 3.5 μm).

Conclusions:

  • The extended acoustical camera provides accurate absolute sizing of microbubbles.
  • This advancement allows for the capture of full radial dynamics of individual microbubbles.
  • The method offers high throughput, analyzing hundreds of microbubbles per hour.