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Updated: Sep 6, 2025
Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro
Published on: March 17, 2023
Protocol for assessing ex vivo lipolysis of murine adipose tissue
Debasish Roy1, Julia M Myers1, Andrea Tedeschi2
1Department of Neuroscience, Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA.
Abstract:
Here, we provide a detailed protocol for assessing ex vivo lipolysis of subcutaneous and visceral white adipose tissue. We describe a robust approach to detect depot-specific changes in lipolytic potential under basal and beta-adrenergic receptor-stimulated conditions. Given that adipose tissue plays a critical role in systemic metabolic health, this experimental protocol can be used to determine changes in adipose tissue function in health and disease.
