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Updated: Sep 6, 2025
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Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells
Published on: November 12, 2015
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Adenovirus infection controls processing bodies to stabilize AU-rich element-containing mRNA
Takeshi Kuroshima1, Aya Yanagawa Matsuda2, Elora Hossain3
1Department of Vascular Biology and Molecular Pathology, Hokkaido University Faculty of Dental Medicine and Graduate School of Dental Medicine, Sapporo, 060-8586, Japan; Department of Oral Diagnosis and Medicine, Hokkaido University Faculty of Dental Medicine and Graduate School of Dental Medicine, Sapporo, 060-8586, Japan.
Virology
|July 2, 2022
Summary
Adenovirus infection causes processing bodies (PBs) to move to aggresomes, preventing the degradation of AU-rich element (ARE)-containing mRNAs. This viral strategy stabilizes ARE-mRNAs, crucial for replication.
Area of Science:
- Molecular Virology
- Cell Biology
- RNA Metabolism
Background:
- Adenovirus infection relies on selective mRNA export for replication.
- AU-rich element (ARE)-containing mRNAs are stabilized in infected cells.
- Ribonucleoprotein (RNP) granules, like processing bodies (PBs), regulate mRNA fate.
Purpose of the Study:
- To elucidate the mechanism of ARE-mRNA stabilization during adenovirus infection.
- To investigate the role of RNP granules, specifically PBs, in this process.
Main Methods:
- Analysis of processing bodies (PBs) and aggresomes in adenovirus-infected cells.
- Utilized adenovirus E4orf3 mutants to assess the requirement for PB translocation.
- Luciferase assays to evaluate the impact on microRNA (miRNA)- and ARE-mediated mRNA decay.
Main Results:
- Processing bodies (PBs) aggregate and translocate to viral aggresomes during late-stage adenovirus infection.
- Adenovirus E4orf3 protein is essential for PB translocation to aggresomes.
- Specific domains of E4orf3, critical for promyelocytic leukemia body morphology, are also vital for PB translocation and mRNA decay inhibition.
Conclusions:
- Adenovirus manipulates PB behavior by translocating them to aggresomes, mediated by E4orf3.
- This viral mechanism effectively prevents the downregulation of ARE-mRNAs.
- The findings reveal a novel strategy by adenovirus to stabilize viral mRNAs, promoting efficient replication.
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