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Updated: Sep 6, 2025

Author Spotlight: Advancing Real-Time cAMP Detection in Cells Using cADDis Biosensor
Published on: March 22, 2024
The epoxy fatty acid pathway enhances cAMP in mammalian cells through multiple mechanisms
Naoki Matsumoto1, Nalin Singh1, Kin Sing Lee2
1Department of Entomology and Nematology, and Comprehensive Cancer Center, University of California, Davis CA, USA.
Abstract:
The cellular mechanism by which epoxy fatty acids (EpFA) improves disease status is not well characterized. Previous studies suggest the involvement of cellular receptors and cyclic AMP (cAMP). Herein, the action of EpFAs derived from linoleic acid (LA), arachidonic acid (ARA), and docosahexaenoic acid on cAMP levels was studied in multiple cell types to elucidate relationships between EpFAs, receptors and cells' origin. cAMP levels were enhanced in HEK293 and LLC-PK1 cells by EpFAs from LA and ARA. Using selective antagonists, the EpFA effects on cAMP levels appear dependent on the prostaglandin E2 receptor 2 (EP2) but not 4 (EP4). Human coronary artery smooth muscle cells responded similarly to the EpFAs. However, we were not able to show the involvement of any of the receptors tested in this cell type. The results pinpointed distinct cell lines and receptor subtypes that natively respond to EpFA.
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