Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Infertility in Males

Infertility in Males

Male infertility affects millions of couples worldwide, arising from various factors that impact different stages of the reproductive process. An endocrine imbalance resulting from conditions like hypogonadism, Klinefelter syndrome, or pituitary disorders can disrupt hormone levels and reduce sperm production. Testicular defects, such as tumors, cryptorchidism, atrophic testes, abnormal sperm morphology, and low sperm count or motility, may arise due to genetic factors, structural...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

PCP protein Prickle 1 regulates Sertoli cell and testis function via cytoskeletal organization through the recruitment of multiple regulatory proteins.

American journal of physiology. Cell physiology·2025

Same author

scRNA-Seq-Based Transcriptome Profiling and Relevant Bioinformatics Approaches to Uncover Novel Insights in Studying Human Spermatogenesis.

Advances in experimental medicine and biology·2025

Same author

Interacting Fat1 and Dchs Planar Cell Polarity Proteins Supported by Fjx1 Serve as Heterodimeric Intercellular Bridges Crucial to Support Spermatogenesis.

Advances in experimental medicine and biology·2025

Same author

Microtubule-Associated Proteins (MAPs) Are Multifunctional Cytoskeletal Proteins in the Testis That Regulate Spermatogenesis.

Advances in experimental medicine and biology·2025

Same author

Testis Is a Sanctuary Site for HIV-1.

Advances in experimental medicine and biology·2025

Same author

Katanin regulatory subunit B1 (KATNB1) regulates BTB dynamics through changes in cytoskeletal organization.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2024

Search research articles

Related Experiment Video

Updated: Sep 6, 2025

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

Published on: February 10, 2023

Human obstructive (postvasectomy) and nonobstructive azoospermia - Insights from scRNA-Seq and transcriptome

Shitao Chen¹, Geng An², Hanshu Wang¹

¹International Peace Maternity and Child Health Hospital, Shanghai Key Laboratory for Reproductive Medicine, School of Medicine, Shanghai Jiaotong University, Shanghai 200030, PR China.

Genes & Diseases

|July 5, 2022

Summary

Investigating azoospermia, this study used single-cell RNA sequencing on testis biopsies. Researchers identified key transcriptional changes in Sertoli cells and germ cell discrepancies, offering insights into male infertility and spermatogenesis.

Keywords:

Androgen signaling Retinoic acid Sertoli cell-only syndrome Single-cell Spermatogenesis

More Related Videos

Mouse Round Spermatid Injection

Mouse Round Spermatid Injection

Published on: January 26, 2024

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients

Published on: November 19, 2019

Related Experiment Videos

Last Updated: Sep 6, 2025

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

Published on: February 10, 2023

Mouse Round Spermatid Injection

Mouse Round Spermatid Injection

Published on: January 26, 2024

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients

Published on: November 19, 2019

Area of Science:

Reproductive Biology
Genomics
Cellular Biology

Background:

Azoospermia is a significant cause of male infertility, yet its molecular underpinnings are poorly understood.
Understanding the cellular and molecular basis of azoospermia is crucial for developing effective treatments.

Purpose of the Study:

To investigate the molecular mechanisms of azoospermia using single-cell RNA sequencing.
To identify transcriptional changes in testicular cells, particularly Sertoli cells, in response to germ cell loss.
To analyze germ cell population discrepancies in obstructive azoospermia.

Main Methods:

Single-cell RNA sequencing (scRNA-seq) of testis biopsy samples from patients with obstructive azoospermia (OA) and nonobstructive azoospermia (NOA).
Comparative analysis of gene expression profiles in OA, NOA, and healthy individuals.
Validation using publicly available datasets (GSM3526588 and GSE124263).

Main Results:

Nonobstructive azoospermia samples contained only somatic cells, indicating a complete absence of germ cells.
Sertoli cells in NOA samples exhibited transcriptional changes related to the loss of germ cell interactions.
A significant germ cell population discrepancy was observed in an OA (postvasectomy) patient compared to a healthy individual.
Regulatory mechanisms at the transcriptional level were identified, providing insights into spermatogenesis disruptions.

Conclusions:

Single-cell RNA sequencing reveals critical molecular insights into azoospermia.
The study highlights the impact of germ cell loss on Sertoli cell function and identifies germ cell population alterations in OA.
Findings contribute valuable information for understanding human spermatogenesis and guiding future research on male reproductive diseases.