Downregulation of hsa_circ_0000885 suppressed osteosarcoma metastasis and progression via regulating E2F3 expression and sponging miR-16-5p
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Updated: Sep 5, 2025
Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
Published on: April 7, 2017
Yanfang Li1, Zhiqing Wu1, Jianlin Shen2
1Department of Pediatric Surgery, Affiliated Hospital of Putian University, #181 Meiyuan East Road, Putian, 351100, Fujian, China.
Introduction:
Accumulating evidence has shown that circular RNAs (circRNAs) have indispensable functions during tumor progression by regulating gene expression. A previous study found that upregulation of hsa_circ_0000885 indicated a poor clinical outcome of osteosarcoma (OS). However, the regulatory mechanism of this process is unclear.
Methods:
This investigation aimed to elucidate how hsa_circ_0000885 regulated OSs. The study used RT-qPCR to investigate hsa_circ_0000885 expression in OS cells. We conducted luciferase reporter assays and analyses to confirm the hsa_circ_0000885 downstream target. We transfected OS cells using different vectors and used Transwell migration, colony formation, western blotting, Matrigel invasion, proliferation, in vivo tumorigenesis, and metastasis assays to identify the role of hsa_circ_0000885 in OS.
Results:
The results showed that hsa_circ_0000885 expression altered OS cell lines, and that hsa_circ_0000885 downregulation suppressed OS cell proliferation and invasion using in vivo and in vitro experiments. Luciferase reporter assays verified that miR-16-5p and E2F3 were downstream targets of hsa_circ_0000885. E2F3 overexpression or miR-16-5p inhibition reversed OS cell invasion and proliferation after silencing hsa_circ_0000885. Furthermore, hsa_circ_0000885 affected cancer stem cell differentiation by regulating miR-16-5p/E2F3.
Conclusions:
Overall, the results showed that hsa_circ_0000885 downregulation suppressed OS progression and metastasis via regulating E2F3 expression and sponging miR-16-5p.
Insights
Downregulation of hsa_circ_0000885 inhibits osteosarcoma (OS) progression and metastasis. This circular RNA regulates E2F3 expression and sponges miR-16-5p, impacting cancer stem cell differentiation.
Area of Science:
- Oncology
- Molecular Biology
- Gene Regulation
Background:
- Circular RNAs (circRNAs) play crucial roles in tumor progression by modulating gene expression.
- Previous studies linked hsa_circ_0000885 upregulation to poor osteosarcoma (OS) outcomes, but its regulatory mechanism remained unclear.
Purpose of the Study:
- To elucidate the regulatory mechanism of hsa_circ_0000885 in osteosarcoma (OS).
- To investigate the role of hsa_circ_0000885 in OS cell proliferation, invasion, and metastasis.
Main Methods:
- RT-qPCR was used to assess hsa_circ_0000885 expression in OS cells.
- Luciferase reporter assays identified downstream targets miR-16-5p and E2F3.
- In vitro and in vivo assays (Transwell, colony formation, western blotting, proliferation, tumorigenesis, metastasis) evaluated the functional role of hsa_circ_0000885.
Main Results:
- hsa_circ_0000885 downregulation suppressed OS cell proliferation and invasion.
- miR-16-5p and E2F3 were confirmed as downstream targets of hsa_circ_0000885.
- E2F3 overexpression or miR-16-5p inhibition reversed the effects of hsa_circ_0000885 silencing on OS cells.
Conclusions:
- hsa_circ_0000885 downregulation inhibits osteosarcoma (OS) progression and metastasis.
- The mechanism involves regulating E2F3 expression and sponging miR-16-5p.
- hsa_circ_0000885 influences cancer stem cell differentiation through the miR-16-5p/E2F3 pathway.
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