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Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells
Published on: September 28, 2018
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Increase recombinant antibody yields through optimizing vector design and production process in CHO cells.
Yongxiao Yang1,2, Zhengmei Li1,2, Qin Li2
1School of Life Science and Technology, Xinxiang Medical University, Xinxiang, China.
Applied Microbiology and Biotechnology
|July 5, 2022
Summary
Optimizing expression vectors and culture conditions in Chinese hamster ovary (CHO) cells significantly boosts recombinant antibody production. A dual promoter vector combined with low-temperature fed-batch culture achieved the highest yields of adalimumab.
Area of Science:
- Biotechnology
- Cell Biology
- Biopharmaceutical Manufacturing
Background:
- Chinese hamster ovary (CHO) cells are the primary hosts for recombinant monoclonal antibody (mAb) production.
- Current strategies often focus on either upstream or downstream processes, limiting overall yield optimization.
Purpose of the Study:
- To enhance recombinant adalimumab expression in CHO cells by integrating vector design and production process optimization.
- To evaluate different expression vector systems (bicistronic, monocistronic, dual promoter) and culture conditions (low-temperature, fed-batch).
Main Methods:
- Construction and comparison of bicistronic, monocistronic, and dual promoter vectors for adalimumab expression.
- Optimization of CHO cell culture using low-temperature and fed-batch strategies.
- Assessment of antibody yield and functional activity (TNF-α binding).
Main Results:
- The dual promoter vector demonstrated superior performance in both transient and stable CHO cell transfections.
- Low-temperature and fed-batch culture conditions further amplified adalimumab yields.
- The purified recombinant adalimumab retained its binding activity against tumor necrosis factor-α (TNF-α).
Conclusions:
- Combining optimized expression vector design (dual promoter) with advanced culture techniques (low-temperature, fed-batch) is crucial for maximizing recombinant mAb production in CHO cells.
- The study highlights the effectiveness of a holistic approach to bioprocess development for improved antibody yields.
