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Real-Time Interfacial Nanothermometry Using DNA-PAINT Microscopy.

Sjoerd W Nooteboom1, Yuyang Wang1, Swayandipta Dey1

  • 1Eindhoven University of Technology, Department of Applied Physics and Institute for Complex Molecular Systems, Eindhoven, 5600 MB, The Netherlands.

Small (Weinheim an Der Bergstrasse, Germany)
|July 5, 2022
PubMed
Summary
This summary is machine-generated.

A new nanothermometer uses DNA interactions to measure local temperatures near nanomaterials, crucial for biomedical applications. This breakthrough allows precise, real-time monitoring of interfacial heating effects on biomolecules.

Keywords:
DNA-mediated points accumulationimaging nanoscale topographynanothermometryparticle heatingplasmon-enhanced fluorescencesingle-molecule microscopy

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Area of Science:

  • Nanotechnology
  • Biomedical Engineering
  • Physical Chemistry

Background:

  • Biofunctionalized nanoparticles are vital for biomedical applications like drug delivery and hyperthermia.
  • Laser excitation can alter nanoparticle-conjugated biomolecules due to localized heating.
  • Existing methods cannot directly measure temperature at the biomolecule-nanomaterial interface.

Purpose of the Study:

  • To develop a novel method for direct, precise measurement of local interfacial temperatures near nanomaterials.
  • To investigate particle-to-particle temperature variations in nanomaterial systems.
  • To enable real-time monitoring of interfacial temperature dynamics.

Main Methods:

  • Utilized DNA-mediated points accumulation for imaging nanoscale topography (DNA-PAINT) microscopy.
  • Leveraged temperature-dependent kinetics of reversible DNA interactions as a nanothermometer.
  • Measured interfacial temperatures of individual gold nanoparticles in parallel.

Main Results:

  • Achieved a precision of 1 K in interfacial temperature measurements.
  • Observed significant particle-to-particle temperature variations, consistent with optical and thermal property differences.
  • Demonstrated real-time temperature tracking with intervals of a few minutes.

Conclusions:

  • The DNA-PAINT nanothermometer accurately measures local interfacial temperatures without prior sample property knowledge.
  • This method provides critical insights into nanomaterial interfacial heating, essential for optimizing biomedical applications.
  • Enables better understanding and control of thermal effects in diverse nanomaterial systems.