Related Experiment Video

Updated: Aug 12, 2026

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
11:12

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material

Published on: August 1, 2018

8.1K

Trophoblast Cell Surface Antigen 2 gene (TACSTD2) expression in primary breast cancer

Neelima Vidula1, Christina Yau2, Hope Rugo2

  • 1Massachusetts General Hospital Cancer Center, 55 Fruit Street, Bartlett Hall Extension 1-213, Boston, MA, USA. nvidula@mgh.harvard.edu.

Breast Cancer Research and Treatment
|July 5, 2022
PubMed
Abstract

Insights

Trophoblast cell surface antigen 2 (TROP2) gene expression occurs across all breast cancer subtypes. TACSTD2 expression correlates with genes promoting tumor growth, supporting TROP2 antibody-drug conjugate investigation in all breast cancer types.

Area of Science:

  • Oncology
  • Genomics
  • Biomarker Research

Background:

  • Trophoblast cell surface antigen 2 (TROP2) is a cell surface glycoprotein implicated in various cancers.
  • A TROP2 antibody-drug conjugate (ADC) has demonstrated efficacy in metastatic triple-negative breast cancer (TNBC).
  • Understanding TACSTD2 gene expression is crucial for evaluating TROP2-targeted therapies in breast cancer.

Purpose of the Study:

  • To investigate Trophoblast cell surface antigen 2 (TROP2) gene (TACSTD2) expression in primary breast cancer.
  • To analyze associations between TACSTD2 expression and tumor characteristics, clinical features, and other gene expressions.
  • To assess the potential of TROP2-targeted therapies across diverse breast cancer subtypes.

Main Methods:

  • TACSTD2 gene expression was analyzed using microarray data from three independent cohorts: I-SPY 1 (n=149), METABRIC (n=1992), and TCGA (n=817).
  • Statistical analyses included Kruskal-Wallis tests for clinical features, Wilcoxon rank sum test for chemotherapy response, Cox proportional hazard models for survival, and Pearson correlations for gene associations.
  • Expression levels were compared across breast cancer subtypes, grades, and correlated with clinical and molecular features.

Main Results:

  • TACSTD2 gene expression was detected in all breast cancer subtypes, exhibiting a broad range.
  • Lower TACSTD2 expression was observed in HER2-positive breast cancer compared to HR+/HER2- and TNBC subtypes.
  • TACSTD2 expression positively correlated with epithelial/adhesion and proliferative genes, but inversely with immune genes, and showed no significant association with chemotherapy response or recurrence-free survival.

Conclusions:

  • TACSTD2 gene is expressed across all breast cancer subtypes, notably in luminal A and TNBC.
  • TACSTD2 expression correlates with genes involved in epithelial transformation, adhesion, and proliferation, suggesting a role in tumor growth.
  • These findings support the exploration of TROP2 antibody-drug conjugates (ADCs) for treating all breast cancer subtypes.
Keywords:
Breast CancerTACSTD2 gene expressionTROP2Triple-negative breast cancer

More Related Videos

Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies07:47

Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies

Published on: September 15, 2023

1.6K
High-Throughput Dissociation and Orthotopic Implantation of Breast Cancer Patient-Derived Xenografts06:06

High-Throughput Dissociation and Orthotopic Implantation of Breast Cancer Patient-Derived Xenografts

Published on: December 20, 2024

725

Related Concept Videos

Tumor Progression02:07

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
Cadherins in Tissue Organization01:19

Cadherins in Tissue Organization

The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...