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Lymphomas: membrane markers and cell flow cytometric diagnosis
Annals of Clinical and Laboratory Science
|January 1, 1987
Summary
Cell flow cytometry (CFC) using monoclonal antibodies and surface immunoglobulin analysis aids in identifying B and T cell lymphomas. However, CFC alone is insufficient for definitive diagnosis of reactive lymphadenopathies or Hodgkin's disease.
Area of Science:
- Immunology
- Hematopathology
- Flow Cytometry
Background:
- Lymphocyte membrane markers, particularly T lymphocyte subsets identified by monoclonal antibodies, reflect biological and functional activity.
- Surface immunoglobulin (sIg) analysis provides a more general identification method for B lymphocytes.
- The use of these markers is proposed to aid in the differential diagnosis of lymphomas.
Purpose of the Study:
- To evaluate monoclonal antibodies (MAbs) and surface immunoglobulin (sIg) analysis via cell flow cytometry (CFC) for identifying and classifying lymphomas.
- To compare CFC findings with histopathologic diagnoses (HPD).
Main Methods:
- Studied 58 patients with lymphomas and benign lymph node disorders.
- Prepared lymph node tissue samples for CFC evaluation.
- Utilized MAbs and sIg analysis with light chain monotypism in CFC.
Main Results:
- CFC alone could not characterize hyperplasias and reactive follicular lymphadenopathies.
- B cell and T cell lymphomas were recognizable and differentiable by MAbs and/or light chain monotypism.
- Hodgkin's disease was not identifiable by CFC due to the absence of specific markers.
Conclusions:
- Cell flow cytometry is a rapid adjunct for lymphoma diagnosis, differentiating B and T cell types.
- Morphologic and clinical information are crucial for diagnostic confirmation.
- CFC with MAbs and sIg is currently insufficient for definitive diagnoses of reactive lymphadenopathies, Hodgkin's disease, or some lymphoma classes.