Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

¹H NMR of Labile Protons: Deuterium (²H) Substitution00:48

¹H NMR of Labile Protons: Deuterium (²H) Substitution

957
This lesson illustrates the role of deuterium substitution in simplifying the NMR spectrum of compounds comprising labile protons. One method employed is the use of deuterium. Amongst the three isotopes of hydrogen, deuterium (2H) has a nucleus composed of one proton and one neutron. When the D2O solvent is added to a pure dry ethanol solution, its labile proton is substituted with deuterium.
957
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

1.2K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.2K
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

529
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
529
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

584
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
584

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

cGAS-IFN-I responses by extracting nuclear DNA from dying cells via nucleocytosis.

Nature communications·2026
Same author

Senescence-associated lysosomal dysfunction impairs cystine deprivation-induced lipid peroxidation and ferroptosis.

Nature communications·2025
Same author

Factors Controlling Diastereoselectivity and Reactivity in the Catalytic Aerobic Carbooxygenation of (E)-2-Fluoro-3-aryl-allyl Nitroacetates.

Chemistry, an Asian journal·2025
Same author

Rational Design Principles for <i>De Novo</i> α-Helical Peptide Barrels with Dynamic Conductive Channels.

Journal of the American Chemical Society·2025
Same author

Raman microscopy of cryofixed biological specimens for high-resolution and high-sensitivity chemical imaging.

Science advances·2024
Same author

Nitrogen signaling factor triggers a respiration-like gene expression program in fission yeast.

The EMBO journal·2024

Related Experiment Video

Updated: Sep 5, 2025

Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering CARS
12:56

Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering CARS

Published on: October 17, 2010

13.8K

Deuterium Raman imaging for lipid analysis.

Syusuke Egoshi1, Kosuke Dodo1, Mikiko Sodeoka1

  • 1Synthetic Organic Chemistry Laboratory, RIKEN Cluster for Pioneering Research, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan; Catalysis and Integrated Research Group, RIKEN Center for Sustainable Resource Science, 2-1, Hirosawa, Wako, Saitama, 351-0198, Japan.

Current Opinion in Chemical Biology
|July 6, 2022
PubMed
Summary

Deuterated fatty acids and Raman microscopy enable label-free analysis of lipids and cellular metabolism. This technique allows for detailed investigation of lipid properties and intracellular activities like de novo lipogenesis.

Keywords:
Deuterium labelingHeavy waterLipid metabolismLong chain fatty acidRaman imaging

More Related Videos

Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions
10:02

Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions

Published on: May 27, 2021

4.1K
Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids
10:42

Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids

Published on: May 12, 2023

1.2K

Related Experiment Videos

Last Updated: Sep 5, 2025

Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering CARS
12:56

Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering CARS

Published on: October 17, 2010

13.8K
Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions
10:02

Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions

Published on: May 27, 2021

4.1K
Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids
10:42

Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids

Published on: May 12, 2023

1.2K

Area of Science:

  • Biochemistry
  • Spectroscopy
  • Cell Biology

Background:

  • Raman microscopy offers label-free detection of endogenous biomolecules.
  • Functional groups like alkynes, nitriles, and C-D bonds serve as effective Raman tags.
  • Deuterated compounds are increasingly utilized for enhanced molecular detection.

Purpose of the Study:

  • To review recent advances in applying deuterated fatty acids with Raman microscopy for lipid analysis.
  • To highlight applications in studying tumor-selective cytotoxicity and intracellular metabolic activities.
  • To discuss methods for analyzing lipid physical properties and de novo lipogenesis.

Main Methods:

  • Utilizing deuterated fatty acids as Raman tags for lipid analysis.
  • Employing Raman microscopy for molecular distribution and property determination.
  • Applying spectral unmixing to analyze C-D vibrational frequencies.
  • Investigating de novo lipogenesis using deuterium-labeled precursors.

Main Results:

  • Demonstrated tumor-selective cytotoxicity of deuterated γ-linolenic acid (GLA).
  • Achieved simultaneous two-color imaging of stearate and oleate using deuterated/protonated alkynes.
  • Enabled detailed analysis of lipid physical properties via C-D bond spectral unmixing.
  • Provided insights into intracellular metabolic activities, including de novo lipogenesis.

Conclusions:

  • Deuterated fatty acids combined with Raman microscopy provide powerful tools for label-free lipid analysis and metabolic studies.
  • This approach facilitates the investigation of complex biological processes at the cellular level.
  • Future applications may include advanced diagnostics and therapeutic monitoring.