Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Valence Bond Theory02:42

Valence Bond Theory

8.9K
Coordination compounds and complexes exhibit different colors, geometries, and magnetic behavior, depending on the metal atom/ion and ligands from which they are composed. In an attempt to explain the bonding and structure of coordination complexes, Linus Pauling proposed the valence bond theory, or VBT, using the concepts of hybridization and the overlapping of the atomic orbitals. According to VBT, the central metal atom or ion (Lewis acid) hybridizes to provide empty orbitals of suitable...
8.9K
Electrostatic Boundary Conditions in Dielectrics01:27

Electrostatic Boundary Conditions in Dielectrics

2.1K
When an electric field passes from one homogeneous medium to another, crossing the boundary between the two mediums imparts a discontinuity in the electric field. This results in electrostatic boundary conditions that depend on the type of mediums the field propagates through.
Consider a case where both the mediums across a boundary are two different dielectric materials. Recall that the electric field and electric displacement are proportional and related through the material's permittivity....
2.1K
Torsion of Noncircular Members01:16

Torsion of Noncircular Members

901
Circular shafts undergoing torsional stress maintain their cross-sectional integrity due to their axisymmetric nature. This symmetry ensures an even distribution of stress, allowing the shaft to withstand torsion without distorting. In contrast, square bars, lacking this axial symmetry, experience significant distortion across their cross-sections when subjected to torsion, with the exception of along their diagonals and at lines connecting midpoints. A detailed examination of a cubic element...
901
Thin-Walled Hollow Shafts01:15

Thin-Walled Hollow Shafts

740
In analyzing a thin-walled hollow shaft subjected to torsional loading, a segment with width dx is isolated for examination. Despite its equilibrium state, this segment faces torsional shearing forces at its ends. These forces are quantitatively described by the product of the longitudinal shearing stress on the segment's minor surface and the area of this surface, leading to the concept of shear flow. This shear flow is consistent throughout the structure, indicating a uniform distribution of...
740
Unsymmetric Bending - Angle of Neutral Axis01:15

Unsymmetric Bending - Angle of Neutral Axis

1.0K
Unsymmetrical bending occurs when a structural member is subjected to bending moments in a plane that does not align with the member's principal axes. This scenario typically arises in beams and other structural components when loads are applied at non-ideal angles, introducing complexities in stress analysis.
When a bending moment is applied at an angle θ concerning the vertical axis of a symmetrical member, it can be resolved into components along the member's principal...
1.0K
Steady, Laminar Flow Between Parallel Plates01:17

Steady, Laminar Flow Between Parallel Plates

1.1K
Understanding steady, laminar flow between parallel plates is essential for analyzing and designing flow in narrow rectangular channels, commonly found in various water conveyance and drainage systems. The Navier-Stokes equations govern fluid motion and are generally challenging to solve due to their nonlinearity. However, simplifications are possible in certain cases, like the steady laminar flow between parallel plates. For this scenario, we assume steady, incompressible, laminar flow.
1.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clocked stepping of an artificial protein walker along a DNA track.

Nature nanotechnology·2026
Same author

<i>De novo</i> grafted coiled-coil peptides as p53/<i>h</i>DM2 inhibitors.

RSC chemical biology·2026
Same author

Kinesin-1 conformational dynamics are controlled by a cargo-sensitive TPR switch.

eLife·2026
Same author

Controlling Nanopore Dynamics via Loop Stapling and Unstapling for Tunable Substrate Transport.

ACS nano·2025
Same author

Orchestrating Self-Replication in Artificial Cells with Digital Microfluidics.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Targeted high-resolution sensing of volatile organic compounds by covalent nanopore detection.

Nature communications·2025

Related Experiment Video

Updated: May 3, 2026

Fabrication and Operation of a Nano-Optical Conveyor Belt
11:10

Fabrication and Operation of a Nano-Optical Conveyor Belt

Published on: August 26, 2015

11.2K

Rational Design Principles for De Novo α-Helical Peptide Barrels with Dynamic Conductive Channels.

Ai Niitsu1, Andrew R Thomson2, Alistair J Scott3

  • 1Laboratory for Dynamic Biomolecule Design, RIKEN Center for Biosystems Dynamics Research, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, Kanagawa 230-0045, Japan.

Journal of the American Chemical Society
|March 28, 2025
PubMed
Summary

Researchers designed coiled coil-based peptides that self-assemble into transmembrane channels. These peptide channels exhibit tunable conductance states, offering a new route for designing functional membrane proteins.

More Related Videos

Design and Optimization Strategies of a High-Performance Vented Box
14:23

Design and Optimization Strategies of a High-Performance Vented Box

Published on: June 9, 2023

1.8K
Development of a Microfluidics-Based Approach for Investigating Microtubule Polymer Mechanics
06:03

Development of a Microfluidics-Based Approach for Investigating Microtubule Polymer Mechanics

Published on: May 30, 2025

970

Related Experiment Videos

Last Updated: May 3, 2026

Fabrication and Operation of a Nano-Optical Conveyor Belt
11:10

Fabrication and Operation of a Nano-Optical Conveyor Belt

Published on: August 26, 2015

11.2K
Design and Optimization Strategies of a High-Performance Vented Box
14:23

Design and Optimization Strategies of a High-Performance Vented Box

Published on: June 9, 2023

1.8K
Development of a Microfluidics-Based Approach for Investigating Microtubule Polymer Mechanics
06:03

Development of a Microfluidics-Based Approach for Investigating Microtubule Polymer Mechanics

Published on: May 30, 2025

970

Area of Science:

  • Biophysics
  • Structural Biology
  • Membrane Protein Engineering

Background:

  • Designing membrane-spanning peptides for specific functions, like forming conductive channels, is complex due to incomplete knowledge of sequence-structure-function relationships.
  • Coiled-coil structures offer a promising framework for building transmembrane peptide assemblies.

Purpose of the Study:

  • To rationally design and characterize novel coiled coil-based peptides that self-assemble into functional transmembrane channels.
  • To investigate the relationship between peptide sequence, barrel geometry, and channel conductance.
  • To establish design principles for engineering tunable peptide-based ion channels.

Main Methods:

  • Utilized a combination of rational design and computational modeling to create peptide sequences.
  • Characterized peptide self-assembly into transmembrane α-helical barrels using biophysical techniques in detergent micelles.
  • Investigated channel activity and conductance states in lipid bilayers using electrophysiology.

Main Results:

  • Successfully designed peptides that form transmembrane α-helical barrels composed of 5 to 7 helices.
  • Observed two distinct conductance states in lipid bilayers: stable low-conductance states dependent on sequence and geometry, and dynamic high-conductance states.
  • High-conductance states were similar across different peptide designs, suggesting the formation of large, dynamic channels analogous to natural barrel-stave channels.

Conclusions:

  • Demonstrated a rational design strategy for creating functional, membrane-spanning peptide channels.
  • Established that peptide sequence and coiled-coil geometry can be tuned to control channel conductance.
  • Provided insights into the assembly and dynamic behavior of peptide channels in lipid bilayers, paving the way for engineered ion channel systems.