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Related Experiment Video

Updated: Sep 5, 2025

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Design of a versatile microfluidic device for imaging precision-cut-tissue slices.

Nafiseh Rafiei1, Mohammadamir G Moghadam1,2, Aaron Au2,3

  • 1Advanced Diagnostics, Toronto General Hospital Research Institute, Toronto, ON, Canada.

Biofabrication
|July 6, 2022
PubMed
Summary

Researchers developed a novel microfluidic device for imaging precision-cut tissues (PCTs). This versatile system enables controlled media exchange and high-resolution 3D fluorescence imaging of living tissue slices.

Keywords:
confocal imaginglight sheet fluorescence microscopy (LSFM)microfluidic deviceprecision-cut-tissue-slice-on-a-chipselective plane illumination microscopy (SPIM)

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Area of Science:

  • Biomedical Engineering
  • Microfluidics
  • 3D Tissue Imaging

Background:

  • Conventional methods for imaging precision-cut tissues (PCTs) often require large reagent volumes and apply ill-defined shear forces.
  • Existing setups hinder the imaging of large volumes and repetitive imaging of specific regions in living tissue slices.

Purpose of the Study:

  • To design a versatile microfluidic device for holding and imaging mouse or human pancreas PCTs.
  • To enable 3D fluorescence imaging using confocal and selective plane illumination microscopy (SPIM).
  • To overcome limitations of conventional imaging methods regarding reagent use, shear force control, and imaging capabilities.

Main Methods:

  • Developed a microfluidic device with a 5 × 5 mm × 140 µm deep chamber and 150 µm tall channels for media exchange.
  • Integrated the device for 3D fluorescence imaging of PCTs using confocal microscopy and SPIM.
  • Controlled localized shear stress on the tissue surface.

Main Results:

  • The microfluidic device facilitates media exchange at approximately 10-fold lower flow rates compared to conventional chambers.
  • The design allows for controlled shear stress application on the tissue.
  • Enabled high-resolution imaging of the same immunofluorescently labeled PCT using both confocal microscopy and SPIM without compromising image quality.

Conclusions:

  • The developed microfluidic device offers a versatile platform for advanced 3D imaging of PCTs.
  • It improves upon conventional methods by reducing reagent consumption, enabling precise shear stress control, and facilitating multi-modal imaging.
  • This technology supports detailed investigation of tissue microenvironments in living slices.