Expression pattern of Stomatin-domain proteins in the peripheral olfactory system
Kevin Y Gonzalez-Velandia1, Andres Hernandez-Clavijo1, Anna Menini2
1Neurobiology Group, SISSA, Scuola Internazionale Superiore di Studi Avanzati, 34136, Trieste, Italy.
Scientific Reports
|July 6, 2022
Summary
Stomatin-like protein 3 (STOML3) and related proteins are present in olfactory sensory neurons, influencing neural activity. This study details their expression and interactions within the olfactory system.
Area of Science:
- Neuroscience
- Molecular Biology
- Cell Biology
Background:
- Stomatin-like protein 3 (STOML3) is found in olfactory sensory neurons (OSNs) and affects neural firing.
- The stomatin family includes STOM, STOML1, STOML2, and podocin, with STOML3, STOM, and STOML1 also present in peripheral sensory neurons where they interact with ion channels.
Purpose of the Study:
- To investigate the expression and potential interactions of STOM and STOML1 alongside STOML3 in OSNs.
- To characterize the presence and localization of other stomatin family members (STOML2, podocin) in the olfactory system.
Main Methods:
- Immunohistochemistry and Western blotting to detect protein expression in olfactory tissues.
- Subcellular localization studies to determine protein compartmentalization within OSNs.
- Co-immunoprecipitation assays to assess protein-protein interactions.
Main Results:
- STOML3, STOM, and STOML1 are expressed in OSNs, with STOML1 at low levels.
- STOML3 and STOM exhibit similar expression patterns, and STOML3 is crucial for STOM's localization to OSN cilia.
- Podocin is absent in the olfactory system, while STOML2 displays a widespread expression pattern across various cell types.
Conclusions:
- This study provides the first comprehensive analysis of the stomatin-domain protein family within the olfactory system.
- Identified precise localization and potential interactions among STOML3, STOM, and STOML1 in OSNs.
- These findings offer insights into the functional roles of stomatin proteins in olfactory sensory neuron activity.
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