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Updated: Sep 5, 2025

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants
07:38

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Published on: June 6, 2025

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SEMA4D/PlexinB1 promotes AML progression via activation of PI3K/Akt signaling

Lu Liu1, Lin Yang1, Xiaojun Liu1

  • 1Department of Hematology, Key Laboratory of Hematology, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000, Hebei, China.

Journal of Translational Medicine
|July 6, 2022
PubMed
Abstract

Insights

SEMA4D protein is elevated in acute myeloid leukemia (AML) and drives cancer growth by activating the PI3K/Akt pathway. Targeting SEMA4D with VX15/2503 antibody shows promise for AML treatment.

Area of Science:

  • Hematology
  • Oncology
  • Molecular Biology

Background:

  • Acute myeloid leukemia (AML) is a prevalent adult leukemia.
  • SEMA4D, a transmembrane protein, is implicated in various solid tumors.
  • The function of SEMA4D in AML remains uninvestigated.

Purpose of the Study:

  • To investigate the role of SEMA4D in AML.
  • To explore SEMA4D as a potential therapeutic target and biomarker in AML.

Main Methods:

  • Utilized cBioPortal and GEPIA for SEMA4D expression and clinical data analysis.
  • Quantified SEMA4D expression via real-time quantitative PCR and western blot.
  • Assessed AML cell proliferation and apoptosis using CCK8 and flow cytometry.

Main Results:

  • SEMA4D expression is upregulated in AML patients, correlating with prognosis.
  • SEMA4D promotes AML cell proliferation and survival by activating the PI3K/Akt pathway via PlexinB1.
  • SEMA4D reduces AML cell sensitivity to daunorubicin.
  • An anti-SEMA4D antibody (VX15/2503) inhibited AML growth in xenograft models.

Conclusions:

  • SEMA4D is a potential predictive biomarker for AML.
  • SEMA4D represents a promising therapeutic target for AML treatment.
Keywords:
Acute myeloid leukemiaPI3K/AktPlexinB1PrognosticSEMA4D

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