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Forward Genetic Approaches in Chlamydia trachomatis
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CT295 Is Chlamydia trachomatis' Phosphoglucomutase and a Type 3 Secretion Substrate.

Sébastien Triboulet1, Maimouna D N'Gadjaga1,2, Béatrice Niragire1

  • 1Institut Pasteur, Université Paris Cité, CNRS UMR3691, Unité de Biologie Cellulaire de l'Infection Microbienne, Paris, France.

Frontiers in Cellular and Infection Microbiology
|July 7, 2022
PubMed
Summary
This summary is machine-generated.

Chlamydia trachomatis bacteria convert stored glycogen into usable glucose-6-phosphate via a bacterial phosphoglucomutase (PGM). This process, facilitated by a type three secretion (T3S) signal, shapes glycogen metabolism in Chlamydiaceae.

Keywords:
Chlamydiaglycogen, metabolismphosphoglucomutase (PGM)secretion signaltype 3 secreted effectors

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Area of Science:

  • Microbiology
  • Bacterial Metabolism
  • Molecular Biology

Background:

  • Obligate intracellular bacteria *Chlamydia trachomatis* store glycogen within host-derived vacuoles.
  • Glycogen breakdown yields glucose-1-phosphate (Glc1P), but *Chlamydia* can only import glucose-6-phosphate (Glc6P).

Purpose of the Study:

  • To investigate the enzymatic conversion of Glc1P to Glc6P within *Chlamydia*-infected vacuoles.
  • To identify the phosphoglucomutase (PGM) responsible for this conversion and explore its origin (host vs. bacterial).

Main Methods:

  • Biochemical assays using purified proteins to test for PGM activity.
  • Complementation studies using PGM-deficient fibroblasts.
  • Bioinformatic analysis of *Chlamydia* CT295 orthologs for secretion signals.

Main Results:

  • Host PGM was not detected within the vacuole.
  • Bacterial protein CT295, but not CT815, demonstrated robust PGM activity.
  • Glycogen accumulation in *Chlamydia* species correlated with the presence of a type three secretion (T3S) signal in CT295 orthologs.

Conclusions:

  • Bacterial PGM (CT295) catalyzes the essential conversion of Glc1P to Glc6P.
  • Acquisition of a T3S signal by CT295 likely influenced the evolution of glycogen metabolism in *Chlamydiaceae*.