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Updated: Sep 5, 2025
Quantification of the Potential Impact of Glyphosate-Based Products on Microbiomes
Published on: January 10, 2022
Development of a method to determine workers' personal exposure levels to glyphosate
Kenta Ishii1,2, Akito Takeuchi1, Osamu Nishinoiri3
1Osaka Occupational Health Service Center, Japan Industrial Safety and Health Association, Osaka, Japan.
Objectives:
We aimed to develop a method to determine workers' personal exposure levels to N-(phosphonomethyl)glycine (glyphosate) for their risk assessments.
Methods:
The proposed method was assessed as follows: recovery, stability of samples on storage, method limit of quantification, and reproducibility. Glyphosate in air was sampled using an air-sampling cassette containing a glass fiber filter. Ultrapure water was used to extract glyphosate from sampler filters. After derivation with 9-fluorenylmethyloxycarbonyl chloride, samples were analyzed by high-performance liquid chromatography using a fluorescence detector.
Results:
Spiked samples indicated an overall recovery of 101%. After 7 days of storage at 4°C, recoveries were approximately 100%. The method limit of quantification was 0.060 μg/sample. Relative standard deviations representing overall reproducibility, defined as precision, were 1.4%-1.8%.
Conclusions:
The method developed in this study allows 4-h personal exposure monitoring of glyphosate at 0.250-500 μg/m3 . Thus, this method can be used to estimate worker exposure to glyphosate.
