Related Experiment Video

Updated: Aug 12, 2026

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method

Published on: October 23, 2018

Separation of two isozymes of polyamine oxidase from murine L1210 leukemia cells

P R Libby, C W Porter

Biochemical and Biophysical Research Communications

|April 14, 1987

Abstract:

Polyamine oxidase from L1210 cells has been separated into two isozymes, A and B on the basis of chromatography on hydroxyapatite. The two isozymes also show different elution characteristics from MGBG Sepharose and Sephacryl-300. The molecular weights of A and B isozymes are estimated to be 260,000 and 200,000 daltons, respectively. The two isozymes show different kinetic characteristics with spermidine, spermine, N1-acetylspermine, and N1,N12-diacetylspermine.

More Related Videos

Isolation and Characterization of Mouse Primary Liver Sinusoidal Endothelial Cells

Isolation and Characterization of Mouse Primary Liver Sinusoidal Endothelial Cells

Published on: December 16, 2021

Cell-Type Specific Protein Purification and Identification from Complex Tissues Using a Mutant Methionine tRNA Synthetase Mouse Line

Cell-Type Specific Protein Purification and Identification from Complex Tissues Using a Mutant Methionine tRNA Synthetase Mouse Line

Published on: April 13, 2022

Related Concept Videos

Articles linked to this work by shared authors, journal, and citation graph.

Polyamine biosynthesis impacts cellular folate requirements necessary to maintain S-adenosylmethionine and nucleotide pools.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2009

Polyamine metabolism and tumorigenesis in the Apc(Min/+) mouse.

Biochemical Society transactions·2007

Polyamine depletion in human melanoma cells leads to G1 arrest associated with induction of p21WAF1/CIP1/SDI1, changes in the expression of p21-regulated genes, and a senescence-like phenotype.

Cancer research·2001

Apoptotic signaling in polyamine analogue-treated SK-MEL-28 human melanoma cells.

Cancer research·2001

Spermidine/spermine n(1)-acetyltransferase catalyzes amantadine acetylation.

Drug metabolism and disposition: the biological fate of chemicals·2001

Spermine deficiency resulting from targeted disruption of the spermine synthase gene in embryonic stem cells leads to enhanced sensitivity to antiproliferative drugs.

Molecular pharmacology·2001

See all related articles