Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrating Executive Function Training with Health Behavior Intervention for Preschool Obesity Prevention in China: A Cluster-Randomized Controlled Trial.

The American journal of clinical nutrition·2026
Same author

Drug-Induced Cuproptosis Defines the Therapeutic Window of Celecoxib in Intervertebral Disc Degeneration via the HSP90-RBX1 Axis.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Efficacy comparison of arthroscopic-assisted uniportal spinal surgery and percutaneous endoscopic discectomy for adjacent segment lumbar disc herniation after fusion surgery.

European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·2026
Same author

Supramolecular self-assembly of an herbal fructan and chitosan hydrogel: analysis of the fructan, gel structures and the molecular interaction mechanisms.

Carbohydrate polymers·2026
Same author

Major risk factors of obesity in China and recommendations for future prevention and control efforts: a systematic review and meta-analysis.

The Lancet regional health. Western Pacific·2026
Same author

Integrating network pharmacology and Mendelian randomization to explore potential targets of Fufang Banmao capsule against non-small cell lung cancer.

Pakistan journal of pharmaceutical sciences·2026

Related Experiment Video

Updated: Sep 5, 2025

Small Molecule Screening and Toxicity Testing in Early-stage Zebrafish Larvae
02:52

Small Molecule Screening and Toxicity Testing in Early-stage Zebrafish Larvae

Published on: March 7, 2025

1.4K

Development a high-throughput zebrafish embryo acute toxicity testing method based on OECD TG 236.

Shisan Xu1,2, Fengyan Chen1,2, Huan Zhang3

  • 1School of Life Sciences, Guangxi Normal University, Guilin, China.

Toxicology Mechanisms and Methods
|July 8, 2022
PubMed
Summary

A new, efficient method for zebrafish embryo acute toxicity testing was developed, improving upon the OECD 236 guideline. This high-throughput approach offers a faster, more effective way to assess chemical toxicity using zebrafish embryos.

Keywords:
OECD 236Zebrafish embryoacute toxicitycomparisonthroughput

More Related Videos

Rapid Evaluation of Toxicity of Chemical Compounds Using Zebrafish Embryos
07:49

Rapid Evaluation of Toxicity of Chemical Compounds Using Zebrafish Embryos

Published on: August 25, 2019

10.8K
Author Spotlight: High-Throughput Toxicity Screening Using Zebrafish Embryo Startle Response Assay
06:25

Author Spotlight: High-Throughput Toxicity Screening Using Zebrafish Embryo Startle Response Assay

Published on: January 12, 2024

1.6K

Related Experiment Videos

Last Updated: Sep 5, 2025

Small Molecule Screening and Toxicity Testing in Early-stage Zebrafish Larvae
02:52

Small Molecule Screening and Toxicity Testing in Early-stage Zebrafish Larvae

Published on: March 7, 2025

1.4K
Rapid Evaluation of Toxicity of Chemical Compounds Using Zebrafish Embryos
07:49

Rapid Evaluation of Toxicity of Chemical Compounds Using Zebrafish Embryos

Published on: August 25, 2019

10.8K
Author Spotlight: High-Throughput Toxicity Screening Using Zebrafish Embryo Startle Response Assay
06:25

Author Spotlight: High-Throughput Toxicity Screening Using Zebrafish Embryo Startle Response Assay

Published on: January 12, 2024

1.6K

Area of Science:

  • Environmental Toxicology
  • Developmental Toxicology
  • Ecotoxicology

Background:

  • The Organization for Economic Co-operation and Development (OECD) Test Guideline (TG) 236 is a standard for zebrafish embryo acute toxicity testing.
  • There is a growing need for efficient and predictive methods for chemical toxicity assessment using zebrafish embryos.

Purpose of the Study:

  • To develop an improved, high-throughput method for zebrafish embryo acute toxicity testing based on the OECD 236 guideline.
  • To enhance testing efficiency while maintaining accuracy in predicting chemical toxicity.

Main Methods:

  • A modified exposure assay was designed using 4-128 cell stage zebrafish embryos in a 96-well microtiter plate.
  • Lethality endpoints were observed at 48-hours postexposure for 32 chemicals across two batches.
  • Statistical analysis, including linear regression and coefficient of variation (CV), was used for comparison.

Main Results:

  • The modified method showed a strong correlation (R² = 0.9717) between logarithmic LC50 values and the OECD 236 method.
  • Intra- and inter-laboratory CVs for LC50 were generally below 30%, indicating good reproducibility.
  • The developed method proved to be less time-consuming and offered higher throughput.

Conclusions:

  • The developed method is a viable, efficient alternative for high-throughput zebrafish embryo acute toxicity testing.
  • This approach can supplement existing methods for chemical toxicity assessment.
  • The findings support the use of this modified assay for broader application in ecotoxicology.