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Bacterial Detection & Identification Using Electrochemical Sensors

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Prototype Digital Lateral Flow Sensor Using Impact Electrochemistry in a Competitive Binding Assay.

Lennart J K Weiß¹, Philipp Rinklin¹, Bhawana Thakur¹

¹Neuroelectronics - Munich Institute of Biomedical Engineering, Department of Electrical and Computer Engineering, Technical University of Munich, Boltzmannstrasse 11, 85748 Garching, Germany.

|July 8, 2022

Summary

This summary is machine-generated.

This study introduces a novel lateral flow assay using stochastic-impact electrochemistry and silver nanoparticles for sensitive biotin detection. This proof-of-concept assay offers a blueprint for future digital diagnostic sensors.

Keywords:

competitive binding assay digital sensing lateral flow sensor silver nanoparticles single-impact electrochemistry

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Area of Science:

Electrochemistry
Nanotechnology
Assay Development

Background:

Lateral flow assays are widely used for rapid diagnostics.
Existing assays can be limited in sensitivity and digital readout capabilities.
Nanoparticles offer unique electrochemical properties for enhanced detection.

Purpose of the Study:

To demonstrate a lateral flow assay concept based on stochastic-impact electrochemistry.
To explore the use of silver nanoparticles as redox-active labels in such assays.
To develop a prototype for detecting free biotin using competitive binding.

Main Methods:

Utilized silver nanoparticles (AgNPs) as redox-active labels.
Developed a membrane-based microfluidic system for competitive binding.
Employed stochastic-impact electrochemistry for nanoparticle detection downstream.
Immobilized streptavidin on latex beads for biotin capture.

Main Results:

Established requirements for using silver nanoparticles as labels.
Demonstrated a prototype assay detecting free biotin via competitive binding.
Successfully registered excess nanoparticles at downstream electrodes.
Validated the concept of nanoimpacts as a readout mechanism.

Conclusions:

A novel lateral flow assay concept using stochastic-impact electrochemistry is demonstrated.
Silver nanoparticles serve as effective redox-active labels for this assay.
The developed prototype provides a foundation for future digital lateral flow sensors.