Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

202
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
202
CRISPR01:59

CRISPR

52.8K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.8K
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.3K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.3K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.1K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction to "Cryo-EM Structure of the FtsH Periplasmic Domain Reveals Functional Dynamics".

ACS chemical biology·2026
Same author

Corrigendum to 'Biosynthesis, characterisation and biocompatibility of a unique and elastomeric medium chain-length polyhydroxyalkanoate for kidney glomerular tissue engineering' [Mater. Today Bio 33 (2025), 101932].

Materials today. Bio·2026
Same author

Bridging cells with devices: emerging reporters and physical interfaces for bacterial biosensors.

Current opinion in biotechnology·2026
Same author

Purified zymogens reveal mechanisms of snake venom metalloproteinase auto-activation.

eLife·2026
Same author

Heparanase inhibition prevents glycocalyx damage and albuminuria in experimental minimal change disease.

Clinical and translational medicine·2026
Same author

PLCε regulates podocyte differentiation and TGF-β1 responses via alteration of SMAD2/SMAD3 ratio.

Cell communication and signaling : CCS·2026

Related Experiment Video

Updated: Sep 5, 2025

The MultiBac Protein Complex Production Platform at the EMBL
13:51

The MultiBac Protein Complex Production Platform at the EMBL

Published on: July 11, 2013

16.1K

Highly efficient CRISPR-mediated large DNA docking and multiplexed prime editing using a single baculovirus.

Francesco Aulicino1, Martin Pelosse1, Christine Toelzer1

  • 1BrisSynBio Bristol Synthetic Biology Centre, Biomedical Sciences, School of Biochemistry, 1 Tankard's Close, University of Bristol, Bristol BS8 1TD, UK.

Nucleic Acids Research
|July 8, 2022
PubMed
Summary

Baculovirus vectors overcome gene editing delivery limits for human cells. This versatile platform enables large DNA payloads for precise gene editing, including complex edits and disease gene correction.

More Related Videos

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
07:46

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

6.0K
Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

22.0K

Related Experiment Videos

Last Updated: Sep 5, 2025

The MultiBac Protein Complex Production Platform at the EMBL
13:51

The MultiBac Protein Complex Production Platform at the EMBL

Published on: July 11, 2013

16.1K
CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
07:46

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

6.0K
Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

22.0K

Area of Science:

  • Molecular Biology
  • Gene Therapy
  • Virology

Background:

  • CRISPR gene editing requires delivering multiple components, challenging traditional viral vectors.
  • Limited cargo capacity of viral vectors hinders advanced genome engineering.

Purpose of the Study:

  • To leverage baculovirus vectors for efficient delivery of CRISPR components in human cells.
  • To overcome cargo capacity limitations for complex gene editing applications.

Main Methods:

  • Utilized baculovirus vectors to deliver Cas9, sgRNA, and donor DNA for gene editing.
  • Performed whole-exon replacement and prime editing in human cells, including patient-derived cells.

Main Results:

  • Achieved up to 30% efficacy in whole-exon replacement with large DNA payloads.
  • Demonstrated cleavage-free prime editing with up to 100% efficiency and no detectable indels.
  • Successfully rescued gene expression in cells from patients with steroid-resistant nephrotic syndrome.

Conclusions:

  • Baculovirus vectors offer a versatile delivery platform for diverse CRISPR technologies.
  • This approach addresses the need for high-capacity delivery systems in genome engineering.
  • Enables precise single-base to multi-gene level genome interventions.