Capillary Electrophoresis Mass Spectrometry: Developments and Applications for Enantioselective Analysis from...

Area of Science:

• Analytical Chemistry
• Separation Science
• Mass Spectrometry

Background:

• Enantioselective analysis using capillary electrophoresis-mass spectrometry (CE-MS) has been established for over 25 years.
• Traditional methods faced challenges with chiral selector compatibility with mass spectrometry and poor detectability of chiral compounds via UV detection.

Purpose of the Study:

• To review the capabilities of chiral CE-MS in addressing limitations of previous analytical approaches.
• To survey MS-compatible chiral selectors and their achievements over the past decade (2010-2020).
• To examine strategies for combining CE and MS, including partial filling, counter-migration, and molecular micelles.

Main Methods:

• Review of literature focusing on chiral CE-MS instrumentation, principles, and requirements.
• Survey of MS-compatible chiral selectors and their applications.
• Analysis of techniques such as electrokinetic chromatography-MS (EKC-MS), micellar electrokinetic chromatography-MS (MEKC-MS), and capillary electrochromatography-MS (CEC-MS).

Main Results:

• Identification of numerous MS-compatible chiral selectors developed in the last decade.
• Demonstration of effective chiral separations for various compounds using moving and fixed selectors in EKC-MS, MEKC-MS, and CEC-MS.
• Critical examination of noteworthy applications and analytical conditions for chiral compound determination.

Conclusions:

• Chiral CE-MS provides elegant and effective solutions for complex chiral analyses.
• Advancements in MS-compatible selectors and separation strategies have significantly enhanced enantioselective analysis.
• Future trends indicate continued development and application of CE-MS for chiral separations.