Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Craspase Protease Activation Is Sensitive to Oncogenic Single-Nucleotide RNA Mismatches.

ACS chemical biology·2026
Same author

Nanoscale Spatial Organization of ARC High- and Low-Order Assemblies at Excitatory Synapses.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Editorial-Dielectrophoresis 2025.

Electrophoresis·2026
Same author

Molecular basis for anti-jumbo phage immunity by AVAST type 5.

Molecular cell·2026
Same author

Pulsed field ablation: Disrupting technology in cardiac electrophysiology.

Heart rhythm·2025
Same author

Actin and vimentin jointly control cell viscoelasticity and compression stiffening.

Molecular biology of the cell·2025

Related Experiment Video

Updated: Sep 5, 2025

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays MEA
09:14

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays MEA

Published on: September 13, 2012

13.6K

Microtrap array on a chip for localized electroporation and electro-gene transfection.

Aswin Muralidharan1, Georg R Pesch1, Hendrik Hubbe1

  • 1Department of Chemical Engineering, Delft University of Technology, van der Maasweg 9, 2629 HZ Delft, the Netherlands.

Bioelectrochemistry (Amsterdam, Netherlands)
|July 10, 2022
PubMed
Summary

We created a microfluidic chip for efficient single-cell electroporation, ensuring high cell viability. This localized electroporation method precisely delivers biomolecules for diverse applications, from bioprocessing to therapeutics.

Keywords:
ElectrotransfectionGene deliveryLocalized electroporationMicrofluidics

More Related Videos

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection
10:34

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

Published on: January 7, 2022

2.9K
Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery
10:51

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

Published on: August 7, 2014

8.7K

Related Experiment Videos

Last Updated: Sep 5, 2025

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays MEA
09:14

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays MEA

Published on: September 13, 2012

13.6K
The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection
10:34

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

Published on: January 7, 2022

2.9K
Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery
10:51

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

Published on: August 7, 2014

8.7K

Area of Science:

  • Biotechnology
  • Cell Biology
  • Microfluidics

Background:

  • Efficient intracellular delivery of biomolecules is crucial for various biological applications.
  • Existing electroporation methods often face challenges with cell viability and precise delivery.
  • Microfluidic devices offer potential for controlled and localized cellular manipulation.

Purpose of the Study:

  • To develop and validate a localized single-cell electroporation chip for high-efficiency, high-viability delivery of exogenous biomolecules.
  • To investigate the mechanism of localized electroporation in a microtrap array.
  • To demonstrate the versatility of the technology for delivering both small and large molecules.

Main Methods:

  • Development of a microfluidic chip featuring a microtrap array for cell immobilization.
  • Application of electric pulses for electrotransfer of biomolecules into trapped cells.
  • Real-time monitoring of biomolecule electrotransfer.
  • Numerical simulations to elucidate the electroporation mechanism.
  • Demonstration using propidium iodide (small molecule) and plasmid DNA (large molecule).

Main Results:

  • High efficiency and high cell viability were achieved in single-cell electroporation.
  • Localized electroporation within microtraps was confirmed as the permeabilization mechanism via simulations.
  • Successful delivery of both small and large molecules, including plasmid DNA for gene expression, was demonstrated.
  • The microtrap technology proved simple and accurate for precise intracellular delivery.

Conclusions:

  • The developed localized single-cell electroporation chip offers a minimally invasive and highly effective method for intracellular delivery.
  • This technology has broad potential applications in bioprocess engineering and therapeutic development.
  • The microfluidic approach enables precise control over biomolecule delivery at the single-cell level.