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Updated: Sep 5, 2025
10:37
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Published on: November 15, 2017
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Targeted proteomics on its way to discovery.
Marta L Mendes1, Gunnar Dittmar1,2
1Proteomics of cellular signalling, Department of Infection and Immunity, Luxembourg Institute of Health, Strassen, Luxembourg.
Proteomics
|July 11, 2022
Summary
Recent advances in highly multiplexed analysis have expanded targeted proteomics capabilities. This allows for broader proteome coverage, impacting biomarker discovery and diagnostics.
Area of Science:
- Proteomics
- Biomarker Discovery
- Analytical Chemistry
Background:
- Traditionally, targeted and discovery proteomics operated on different detection scales.
- Targeted proteomics focused on limited sets of proteins, while discovery proteomics aimed for broader identification.
- Advances in highly multiplexed analysis have blurred these distinctions.
Purpose of the Study:
- To highlight the evolving capabilities of targeted proteomics.
- To discuss the impact of these advancements on biomarker discovery.
- To explore the potential of targeted proteomics in diagnostics.
Main Methods:
- Highly multiplexed analysis techniques in proteomics.
- Comparative analysis of targeted and discovery proteomics approaches.
- Assessment of dynamic range limitations in proteome analysis.
Main Results:
- Targeted proteomics now covers larger portions of proteomes, including those with low protein abundance.
- Advancements enable analysis of complex proteomes previously challenging for targeted methods.
- Discovery proteomics continues to increase the number of identified and quantified proteins.
Conclusions:
- Highly multiplexed targeted proteomics is expanding its reach into less complex or dynamic-range-restricted proteomes.
- These developments significantly enhance the potential for biomarker discovery.
- Targeted proteomics is becoming increasingly viable for diagnostic applications.
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