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Eleven shades of PASEF.

Marta L Mendes1, Klara F Borrmann1,2, Gunnar Dittmar1,2

  • 1Department of Infection and Immunity, Luxembourg Institute of Health, Strassen, Luxembourg.

Expert Review of Proteomics
|October 22, 2024
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Summary

Trapped ion mobility spectrometry (TIMS) coupled with mass spectrometry, particularly using Parallel Accumulation-Serial Fragmentation (PASEF), significantly enhances protein identification and quantification in proteomics. This technology is advancing clinical proteomics applications.

Keywords:
DIAPASEFParallel Accumulation-Serial FragmentationProteomicsTIMS-TOFion mobilitytargeted proteomics

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Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Biotechnology

Background:

  • Trapped ion mobility spectrometry (TIMS) combined with fast high-resolution time-of-flight (TOF) mass spectrometry has revolutionized proteomics.
  • Parallel Accumulation-Serial Fragmentation (PASEF) is a key technology driving advancements in protein identification and quantification.
  • PASEF has been adapted for both discovery and targeted proteomics applications.

Purpose of the Study:

  • To review the evolution and current state of PASEF measurement techniques.
  • To highlight the application of these techniques in clinical proteomics.
  • To discuss the impact of dual TIMS tunnels on proteomics depth and speed.

Main Methods:

  • Literature search using PubMed and Google Scholar.
  • Review of eleven newly implemented PASEF measurement techniques over the last decade.
  • Analysis of PASEF applications in clinical proteomics.

Main Results:

  • PASEF technology has led to a significant increase in protein identifications and quantifications.
  • The limit of detection for proteins in biological samples has been lowered.
  • Dual TIMS tunnels have enhanced the depth and speed of proteomics measurements.

Conclusions:

  • PASEF techniques have continuously been optimized, leading to eleven new measurement methods.
  • These advancements are significantly pushing the field of clinical proteomics forward.
  • The dual TIMS tunnel approach is revolutionizing proteomics analysis.