Related Experiment Video
Updated: Sep 5, 2025
mRNA Interactome Capture from Plant Protoplasts
Published on: July 28, 2017
Efficient Protein Extraction Protocols for NanoLC-MS/MS Proteomics Analysis of Plant Tissues with High Proteolytic
Esaú Bojórquez-Velázquez1, José M Elizalde-Contreras1, Jesús Alejandro Zamora-Briseño2
1Red de Estudios Moleculares Avanzados, Clúster Científico y Tecnológico BioMimic®, Instituto de Ecología A.C. (INECOL), Xalapa, Veracruz, Mexico.
Abstract:
Proteomics is an essential tool to uncover the regulatory processes of fruit biology. In fruits with high proteolytic activity, the inhibition of endogenous proteases is key for successful protein extraction. In this chapter, we describe an efficient protocol for total protein extraction to deal with this inconvenience using pineapple pulp as an example. We corroborated the efficacy of our protein extraction protocols by carrying out nano LC-MS/MS analyses using a highly sensitive hybrid mass spectrometer. In doing so, we were able to identify over 3000 proteins in pineapple pulp. Our contribution paves the way for massive comparative proteomics scrutiny in pineapple fruits, as well as others plant tissues with high protease activity such as papaya, fig, and kiwi fruits.
