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Updated: Sep 5, 2025
Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
Published on: July 28, 2017
DNA Extraction Methods to Obtain High DNA Quality from Different Plant Tissues
Domenico Rizzo1, Daniele Da Lio2, Linda Bartolini1
1Laboratory of Phytopathological Diagnostics and Molecular Biology, Plant Protection Service of Tuscany, Pistoia, Italy.
Abstract:
DNA extraction from plant samples is very important for a good performance of diagnostic molecular assays in phytopathology. The variety of matrices (such as leaves, roots, and twigs) requires a differentiated approach to DNA extraction. Here we describe three categories of matrices: (a) symptomatic bark/wood tissue; (b) residues of frass resulting from insect woody trophic activities, portions of the galleries produced in the wood, and tissues surrounding exit holes; and (c) leaves of different plant species. To improve the performances of diagnostic assays, we here describe DNA extraction procedures that have been optimized for each matrix type.
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