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A Detached Leaf Assay for Rapidly Screening Plant Pathogen-Biological Control Agents
Giovana Prado Fortuna Macan1, Sammar Khalil2, Pruthvi B Kalyandurg1
1Department of Plant Breeding, Swedish University of Agricultural Sciences, Alnarp, Sweden.
The detached leaf assay (DLA) effectively screens biological control agents (BCAs) for plant diseases. This method rapidly assesses BCAs
Area of Science:
- Plant pathology
- Agricultural science
- Microbiology
Background:
- The detached leaf assay (DLA) is a nondestructive method for evaluating plant-pathogen interactions.
- It allows rapid characterization of pathogen infectivity and plant resistance.
Purpose of the Study:
- To demonstrate the utility of the detached leaf assay (DLA) for assessing biological control agents (BCAs).
- To screen potential BCAs for Phytophthora cactorum, a strawberry pathogen.
Main Methods:
- Utilized the detached leaf assay (DLA) methodology.
- Applied the assay to screen BCAs against Phytophthora cactorum on strawberry (Fragaria × ananassa) leaves.
Main Results:
- Successfully demonstrated the applicability of the DLA for screening BCAs.
- The DLA proved effective in assessing BCAs' potential against P. cactorum.
Conclusions:
- The detached leaf assay (DLA) is a valuable tool for evaluating biological control agents.
- This method accelerates the screening process for BCAs in plant disease management.
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