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Updated: Sep 5, 2025

Lipid Bilayer Vesicle Generation Using Microfluidic Jetting
Published on: February 21, 2014
Generation of Hybrid Extracellular Vesicles by Fusion with Functionalized Liposomes
Max Piffoux1,2,3,4, Amanda Karine Andriola Silva1, Florence Gazeau1
1Laboratoire Matières et Systèmes Complexes, CNRS UMR 7057, Université de Paris, Paris, France.
Abstract:
Extracellular vesicles (EVs) and liposomes are natural and synthetic drug delivery systems, respectively, with their own advantages and limitations. EV/liposome fusion allows the generation of hybrid EVs that benefit from both the versatility of liposomes (tunable lipid and protein composition, surface functionalization, lumen loading, etc.) and the functionality of EVs (natural targeting properties, low immunogenicity, anti-inflammatory properties, etc.). Here, we describe the methods to (1) produce EVs and liposomes, (2) induce and monitor their fusion, and (3) purify the obtained hybrid EVs.
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