Three Different Rapidly Prototyped Polymeric Substrates With Interdigitated Electrodes for Escherichia coli Sensing:...

Area of Science:

• Biosensors and Microdevices
• Electrochemical Sensing
• Pathogen Detection

Background:

• Miniaturized devices are crucial for rapid pathogen detection.
• Interdigitated electrodes (IDEs) offer a platform for electrochemical sensing.
• Escherichia coli (E. coli) is a significant indicator of bacterial contamination.

Purpose of the Study:

• To develop and compare miniaturized IDE sensors for E. coli detection.
• To evaluate fabrication techniques including laser-induced graphene (LIG), direct laser writing, and 3D printing.
• To assess the sensitivity and performance of different IDE sensor designs.

Main Methods:

• Fabrication of IDEs using LIG on polyamide, direct laser writing on glass, and polymeric 3D printing.
• Electrochemical impedance spectroscopy (EIS) in the frequency range of 1 Hz to 1 MHz.
• Quantification of E. coli based on changes in electrical impedance.

Main Results:

• LIG-based IDE sensors demonstrated higher sensitivity compared to other methods.
• Impedance changes of approximately 2.5 [Formula: see text] per E. coli cell doubling were observed.
• The developed microdevices showed potential for microscale pathogenic detection.

Conclusions:

• LIG-based IDEs are a promising approach for sensitive E. coli detection.
• Miniaturized sensors offer a fast and flexible platform for clinical and biomedical analysis.
• This technology facilitates advancements in rapid pathogenic detection systems.