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Updated: Sep 5, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
Published on: May 25, 2018
Applications of CRISPR-Cas9 as an Advanced Genome Editing System in Life Sciences
Kamand Tavakoli1, Alireza Pour-Aboughadareh2, Farzad Kianersi3
1Department of Medical Laboratory Science, Kermanshah Branch, Islamic Azad University, Kermanshah P.O. Box 6718997551, Iran.
Abstract:
Targeted nucleases are powerful genomic tools to precisely change the target genome of living cells, controlling functional genes with high exactness. The clustered regularly interspaced short palindromic repeats associated protein 9 (CRISPR-Cas9) genome editing system has been identified as one of the most useful biological tools in genetic engineering that is taken from adaptive immune strategies for bacteria. In recent years, this system has made significant progress and it has been widely used in genome editing to create gene knock-ins, knock-outs, and point mutations. This paper summarizes the application of this system in various biological sciences, including medicine, plant science, and animal breeding.
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