Related Experiment Video
Updated: Sep 4, 2025
12:26
Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
Published on: May 3, 2018
18.8K
Dynamic cell cycle-dependent phosphorylation modulates CENP-L-CENP-N centromere recruitment
Alexandra P Navarro1,2, Iain M Cheeseman1,2
1Whitehead Institute for Biomedical Research, Cambridge, MA 02142.
Molecular Biology of the Cell
|July 13, 2022
Summary
Phosphorylation of CENP-L and CENP-N regulates the CENP-LN complex
Area of Science:
- Cell Biology
- Molecular Biology
- Genetics
Background:
- The kinetochore is crucial for accurate chromosome segregation during cell division.
- The constitutive centromere-associated network (CCAN) forms the kinetochore platform.
- CCAN assembly, regulation, and dynamics are not fully understood.
Purpose of the Study:
- To investigate the cell cycle-dependent regulation of the CENP-LN complex.
- To determine the role of CENP-L and CENP-N phosphorylation in CENP-LN localization and function.
Main Methods:
- Analysis of CENP-L and CENP-N phosphorylation.
- Investigating CENP-LN complex formation and localization.
- Studying the impact of altered phosphorylation on chromosome segregation.
Main Results:
- Phosphorylation of CENP-L and CENP-N controls CENP-LN complex formation and localization.
- Mimicking constitutive phosphorylation or preventing it disrupts CENP-LN localization.
- Disrupted CENP-LN localization leads to chromosome segregation errors.
Conclusions:
- Phosphorylation cycles of CENP-L and CENP-N are essential for CENP-LN complex dynamics.
- This regulation is critical for CCAN reorganization at kinetochores during the cell cycle.
Related Concept Videos
02:25Centrosome Duplication
4.1K
The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
4.1K
02:39Positive Regulator Molecules
5.6K
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
5.6K
02:15M-Cdk Drives Transition Into Mitosis
5.7K
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
5.7K
00:50Anaphase Promoting Complex
2.9K
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
2.9K
02:34Inhibition of Cdk Activity
4.9K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.9K
02:30Histone Variants at the Centromere
4.5K
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
4.5K