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Enzyme-Linked Immunosorbent Assay01:33

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Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics
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Enzyme-nanozyme cascade colorimetric sensor platform: a sensitive method for detecting human serum creatinine.

Jing Zhu1, Jie Pan2, Yingchun Li3

  • 1Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi, China.

Analytical and Bioanalytical Chemistry
|July 13, 2022
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Summary

This study introduces a novel enzyme-nanozyme cascade sensor for visual creatinine detection. The platform utilizes bismuth ferrite nanozymes and natural enzymes for sensitive and selective detection of creatinine in human serum.

Keywords:
Cascade reactionColorimetryCreatininePerovskite oxidePeroxidase mimics

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Area of Science:

  • Biomarker Detection
  • Nanomaterials Science
  • Enzyme Catalysis

Background:

  • Creatinine is a vital biomarker for kidney, thyroid, and muscle diseases.
  • Accurate creatinine detection is crucial for diagnosing various health conditions.
  • Existing detection methods may lack sensitivity, selectivity, or visual output.

Purpose of the Study:

  • To develop a visual sensing platform for creatinine detection.
  • To utilize an enzyme-nanozyme cascade system for enhanced sensitivity and selectivity.
  • To employ bismuth ferrite (BiFeO3) as a nanozyme with peroxidase-like activity.

Main Methods:

  • Synthesized BiFeO3 perovskite oxide via sol-gel method as a nanozyme.
  • Employed a cascade reaction involving creatinase, creatininase, and sarcosine oxidase.
  • Utilized the nanozyme to catalyze the oxidation of hydrogen peroxide, leading to a color change in TMB.

Main Results:

  • Achieved visual quantification of creatinine.
  • Demonstrated a broad detection range of 0.5–150 μM with a low detection limit of 0.09 μM.
  • Exhibited high selectivity for creatinine in human serum samples, even with interfering substances.

Conclusions:

  • The developed enzyme-nanozyme cascade platform offers a sensitive, selective, and visual method for creatinine detection.
  • The BiFeO3 nanozyme exhibits excellent catalytic efficiency and stability.
  • The platform's design is adaptable for detecting other biomarkers by altering the enzyme combination.