Fluorescent aptamer-modified mesoporous silica nanoparticles for quantitative acetamiprid detection

Area of Science:

• Environmental Chemistry
• Analytical Chemistry
• Nanotechnology

Background:

• Acetamiprid (ACE) is a widely used insecticide that persists in the environment.
• ACE residues pose risks of acute and chronic toxicity to humans and ecosystems.
• A need exists for rapid, sensitive, and convenient methods to quantify ACE.

Purpose of the Study:

• To develop a novel fluorescence-based assay for the rapid detection of acetamiprid.
• To utilize aminated mesoporous silica nanoparticles (MSNs-NH2) and aptamers for ACE quantification.
• To establish a sensitive and selective method for pesticide residue analysis.

Main Methods:

• Synthesis of aminated mesoporous silica nanoparticles (MSNs-NH2) via a one-pot method.
• Adsorption of fluorescein-modified ACE aptamers (FAM-Apt) onto MSNs-NH2 through electrostatic interactions.
• Development of a fluorescence assay based on the displacement of FAM-Apt by ACE.

Main Results:

• The assay demonstrated a linear detection range for ACE from 50-1100 ng/mL.
• A low limit of detection (LOD) of 30.26 ng/mL was achieved.
• The method showed high sensitivity, selectivity, and reproducibility for ACE detection.

Conclusions:

• A simple, sensitive, and selective fluorescence method for ACE detection was successfully established.
• The developed assay is suitable for practical analysis of pesticide residues.
• This approach provides a valuable tool for rapid monitoring of environmental contaminants.