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Related Concept Videos

Canonical Wnt Signaling Pathway02:54

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The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
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Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
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Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
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Gene expression in prokaryotes is governed by constitutive and regulated systems, allowing cells to balance the production of essential proteins with adaptive responses to environmental changes.Constitutive Gene ExpressionConstitutive, or housekeeping, genes are continuously expressed as they encode proteins vital for fundamental cellular processes. These include enzymes for glycolysis, ribosomal components for protein synthesis, and proteins involved in DNA replication. Their constant...
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Related Experiment Video

Updated: Sep 4, 2025

The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions
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Integrated analysis of Wnt signalling system component gene expression.

Paula Murphy1, Chris Armit2, Bill Hill2

  • 1School of Natural Sciences, Department of Zoology, Trinity College Dublin, The University of Dublin, Dublin 2, Ireland.

Development (Cambridge, England)
|July 14, 2022
PubMed
Summary
This summary is machine-generated.

This study maps Wnt and Fzd gene expression in 3D mouse embryos. It reveals novel spatial organization of these key developmental signaling pathways.

Keywords:
3D imagingComputational analysisIntegrated analysisWnt signalling

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Wnt signaling is crucial for embryonic development, controlling tissue patterning and differentiation.
  • This pathway involves complex interactions of ligands, receptors, and modulators encoded by multi-gene families.
  • Understanding Wnt gene expression dynamics is essential for deciphering developmental processes.

Purpose of the Study:

  • To perform an integrated 3D spatial analysis of key Wnt signaling genes in the developing mouse embryo.
  • To map and visualize gene expression patterns across multiple developmental stages.
  • To identify novel territories of gene co-expression and unique expression patterns.

Main Methods:

  • Applied 3D/3D image transformation to map gene expression to a reference embryo.
  • Integrated visual and volumetric analysis of gene expression data.
  • Computational interrogation of combined expression patterns for Wnt and Fzd genes.

Main Results:

  • Identified specific embryonic territories with co-expression of multiple Wnt and Fzd genes.
  • Mapped expression patterns of all seven Wnt paralogous gene pairs.
  • Discovered regions with no detectable Wnt or Fzd expression, and regions with unique single Wnt gene expression.

Conclusions:

  • Revealed a previously unappreciated level of spatial organization in Wnt signaling gene expression.
  • Provides a valuable 3D resource for whole-system analysis of developmental signaling.
  • Offers new insights into the regulatory mechanisms of embryonic patterning and differentiation.