Quantification of Isomaltulose in Food Products by Using Heteronuclear Single Quantum Coherence NMR-Experiments

Isomaltulose is a commonly used sweetener in sports nutrition and in products intended for consumption by diabetics. Because previously established chromatographic methods for quantification of isomaltulose suffer from long analysis times (60-210 min), faster quantitative approaches are required. Here, an HSQC (heteronuclear single quantum coherence) experiment with reduced interscan delay was established in order to quantify isomaltulose next to potential additional sugars such as d-glucose, d-fructose, d-galactose, sucrose, lactose, and maltose in 53 min. By using HSQC coupled to non-uniform sampling (NUS) as well as ASAP-HSQC (acceleration by sharing adjacent polarization), analysis times were reduced to a few minutes. Application of NUS-HSQC with reduced interscan delay takes 27 min, resulting in accurate and precise data. In principle, application of ASAP-HSQC approaches (with analysis times as low as 6 min) can be used; however, precision data may not suffice all applications.