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Gluconeogenesis from acetone in diabetic rats.
Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Metabolisme
|April 1, 1987
Summary
Acetone is a minor source of glucose in rats, but its conversion increases in diabetic animals. This study quanties acetone
Area of Science:
- Biochemistry
- Metabolic pathways
- Animal models
Background:
- Acetone is recognized as a minor precursor for glucose synthesis in vivo.
- Understanding acetone's role in glucose metabolism is crucial, especially in metabolic disorders like diabetes.
Purpose of the Study:
- To quantify the in vivo conversion of acetone to glucose in normal and diabetic rats.
- To investigate the impact of diabetes on the acetone-to-glucose metabolic pathway.
Main Methods:
- Utilized isotopic labeling to trace carbon atoms from acetone to glucose.
- Measured the turnover rate of glucose in both normal and diabetic rat models.
- Analyzed the labeling of plasma lactate in relation to glucose.
Main Results:
- In diabetic rats, 1.30% of circulating glucose carbon originated from acetone, compared to 0.67% in normal rats.
- Acetone-carbon conversion to glucose-carbon was higher in diabetic rats (48 µg/kg·min) than in normal rats (16 µg/kg·min).
- Plasma lactate labeling was consistently higher than glucose labeling in both groups.
Conclusions:
- The transfer of carbon atoms from acetone to glucose is significantly increased in diabetic conditions.
- Acetone remains a quantitatively minor contributor to glucose production, even in the context of diabetic ketonemia.