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Expression of CD3 and CD20 in Antistreptolysin-O Titer Seropositive and Seronegative Children with Chronic
Abeer A K Mohamed1,2, Fahd A Alharbi3, Sahar Khalil1,4
1Department of Histology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
Insights
Children with chronic tonsillitis and high antistreptolysin O titer (ASOT) show increased T-lymphocyte (CD3) and B-lymphocyte (CD20) markers in tonsils. This suggests a link between ASOT levels and immune cell activity in tonsillitis.
Area of Science:
- Pediatric immunology
- Otolaryngology
- Pathology
Background:
- Chronic tonsillitis (CT) is a prevalent pediatric inflammatory condition.
- Serum antistreptolysin O titer (ASOT) is a key diagnostic marker for CT and a factor in tonsillectomy decisions.
Purpose of the Study:
- To investigate the expression of tonsillar T-lymphocyte (CD3) and B-lymphocyte (CD20) markers.
- To correlate these markers with ASOT levels (seropositive vs. seronegative) in pediatric CT cases.
Main Methods:
- Thirty children (6-10 years) with CT were divided into seropositive (ASOT ≥400 IU) and seronegative (ASOT <400 IU) groups.
- Tonsillectomy specimens underwent histopathological examination and immunohistochemical analysis for CD3 and CD20 expression.
Main Results:
- The seropositive ASOT group exhibited significant histopathological changes, including epithelial hyperplasia, abscesses, and lymphocytic infiltration.
- Immunohistochemistry revealed marked CD3 and CD20 expression in the seropositive group, contrasting with mild expression in the seronegative group.
Conclusions:
- Seropositive ASOT in pediatric chronic tonsillitis is associated with distinct histopathological findings.
- Elevated ASOT levels correlate with increased T-lymphocyte (CD3) and B-lymphocyte (CD20) expression in tonsillar tissues.
Background:
Chronic tonsillitis (CT) is a common inflammatory illness in children, and serum antistreptolysin O titer (ASOT) is a common investigation performed for these cases and considered a perfect sign for tonsillectomy.
Objective:
To evaluate the expression of tonsillar T-and B-lymphocytes markers in relation to seropositive or seronegative ASOT in cases of CT.
Materials And Methods:
Thirty children (15 males and 15 females) aged 6-10 years were divided equally into two groups: Group A seropositive ASOT (≥400 IU) and Group B seronegative ASOT (<400 IU). Both performed bilateral tonsillectomy. Specimens from the removed tonsils were taken and prepared for light microscopic examination and immunohistochemical evaluation of CD20 and CD3 expression.
Results:
Seropositive ASOT group showed significant histopathologic changes in the form of hyperplasia of the stratified squamous nonkeratinized epithelium, Urgas's abscess, and severe lymphocytic infiltration. Immunohistochemical results of seropositive ASOT group showed marked expression of CD3 and CD20, while seronegative ASOT group showed mild expression of CD3 and CD20.
Conclusion:
Seropositive ASOT CT, in addition to histopathological changes, is associated with significant increase in both B-lymphocytes (CD20 expression) and T-lymphocytes (CD3 expression) markers.
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