Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.3K
Labeling DNA Probes03:31

Labeling DNA Probes

8.3K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.3K
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

724
Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
724
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.2K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tension TRAAKer: a chemigenetic fluorescent membrane tension reporter.

bioRxiv : the preprint server for biology·2026
Same author

A chemical-genetic approach to target voltage-sensitive fluorophores to mitochondria.

Mitochondrial communications·2026
Same author

Discovery of Coronavirus Main Protease Inhibitors with Enhanced Brain Exposure and Potent Oral Efficacy in SARS-CoV-2 and MERS Infection Models.

Journal of medicinal chemistry·2026
Same author

Membrane potential and feedback dynamics regulate CatSper-mediated progesterone signaling in human sperm.

bioRxiv : the preprint server for biology·2025
Same author

Chemical and ribosomal synthesis of atropisomeric and macrocyclic peptides with embedded quinolines.

Nature chemistry·2025
Same author

High Yield, Low Magnesium Flexizyme Reactions in a Water-Ice Eutectic Phase.

Biochemistry·2025
Same journal

Switching Site Selectivity in Alkoxyamine Hydration: From Lone-Pair Direction to Solvent Network Dominance.

Journal of the American Chemical Society·2026
Same journal

A Topotactic Leap: 2D Layers to 3D Large-Pore Zeolite.

Journal of the American Chemical Society·2026
Same journal

Enhanced Hydrogen Evolution over Single-Atom Catalysts via Electrostatic Polarization in Contact-electro-catalysis.

Journal of the American Chemical Society·2026
Same journal

Tumor Acidity-Activatable Ionizable Lipid Nanoparticles for Selective Oncolytic Therapy.

Journal of the American Chemical Society·2026
Same journal

Alternating Magnetic Field Promotes Ammonia Cracking by Disrupting the Sabatier Limitation of Ruthenium Catalytic Species.

Journal of the American Chemical Society·2026
Same journal

Bulk Ferromagnetic Icosahedral Quasicrystals without Rapid Quenching.

Journal of the American Chemical Society·2026
See all related articles

Related Experiment Video

Updated: Sep 4, 2025

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors
09:57

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors

Published on: February 4, 2016

10.8K

Flipping the Switch: Reverse-Demand Voltage-Sensitive Fluorophores.

Jack T McCann, Brittany R Benlian, Susanna K Yaeger-Weiss

    Journal of the American Chemical Society
    |July 14, 2022
    PubMed
    Summary
    This summary is machine-generated.

    Researchers developed new voltage-sensitive fluorophores (VF dyes) using donor-excited photoinduced electron transfer (d-PeT). These dyes show increased fluorescence with hyperpolarizing membrane potential, offering a novel tool for biological research.

    More Related Videos

    Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
    08:39

    Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

    Published on: December 22, 2020

    4.3K
    Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking
    09:59

    Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking

    Published on: March 16, 2017

    8.9K

    Related Experiment Videos

    Last Updated: Sep 4, 2025

    Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors
    09:57

    Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors

    Published on: February 4, 2016

    10.8K
    Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
    08:39

    Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

    Published on: December 22, 2020

    4.3K
    Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking
    09:59

    Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking

    Published on: March 16, 2017

    8.9K

    Area of Science:

    • Biophysics
    • Chemical Biology
    • Fluorescence Microscopy

    Background:

    • Fluorescence microscopy utilizes environmentally responsive reporters for biological interrogation.
    • Photoinduced electron transfer (PeT) is a key mechanism modulating fluorescence based on environmental changes.
    • Voltage-sensitive fluorophores (VF dyes) are crucial for studying membrane potential (Vm).

    Purpose of the Study:

    • To investigate donor-excited PeT (d-PeT) mechanisms in VF dyes.
    • To design and synthesize novel VF dyes with reversed voltage sensitivity compared to existing acceptor PeT (a-PeT) dyes.
    • To explore the relationship between molecular wire properties and voltage sensitivity.

    Main Methods:

    • Computational modeling and experimental synthesis of VF dyes.
    • Design of VF dyes incorporating electron-withdrawing molecular wires to facilitate d-PeT.
    • Characterization of voltage sensitivity in synthesized VF dyes.

    Main Results:

    • Successfully designed and synthesized five d-PeT VF dye candidates.
    • Two novel d-PeT VF dyes exhibited voltage-sensitive fluorescence.
    • These new dyes display fluorescence increases upon hyperpolarization, a reversal of polarity compared to a-PeT dyes.

    Conclusions:

    • Donor-excited PeT is a viable mechanism for creating VF dyes with reversed voltage sensitivity.
    • The introduction of electron-withdrawing groups effectively modulates VF dye response to membrane potential.
    • This work expands the toolkit of VF dyes for advanced biological imaging and biophysical studies.