Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

Cleaning, Sterilization, and Disinfection

Cleaning, Sterilization, and Disinfection

Cleaning, disinfection, and sterilization are the methods that help to break the infection chain and prevent disease.
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...

Key Techniques in Microbiology

Key Techniques in Microbiology

Aseptic techniques prevent contamination, ensure experimental accuracy, and protect researchers and microbial cultures. These techniques are essential in clinical, industrial, and research settings where sterility is required.Maintaining Sterility in Laboratory PracticesScientists maintain sterility by sterilizing tools with heat or chemicals, disinfecting work surfaces, and handling cultures in controlled environments. Working near an open flame or within a laminar flow hood reduces the risk...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Efficacy of a 20:1 CBD:THC cannabis herbal extract for pain and inflammation in dogs following tibial plateau leveling osteotomy.

Frontiers in veterinary science·2025

Same author

REV1 inhibition enhances trinucleotide repeat mutagenesis.

bioRxiv : the preprint server for biology·2025

Same author

A pandemic risk index to improve supply chains decision-making between US and Mexico: A COVID-19 case study.

PloS one·2025

Same author

Interleukin-27-producing cells in gram-negative neonatal sepsis display diverse phenotypes and functions in the liver.

ImmunoHorizons·2025

Same author

Oral live attenuated polio vaccines induce enhanced T-cell responses with broad antigen recognition compared to inactivated polio vaccines.

medRxiv : the preprint server for health sciences·2025

Same author

FLASH radiotherapy spares lymphocytes in tumor-draining lymph nodes and increases infiltration of immune cells in tumors.

bioRxiv : the preprint server for biology·2025

Same journal

A Rapid Method for Accurately Determining Lipid Nanoparticle Size Using Nano-Flow Cytometry.

Journal of biomolecular techniques : JBT·2026

Same journal

Improving core facility service discovery with an AI assistant grounded in institutional web content.

Journal of biomolecular techniques : JBT·2026

Same journal

ABRF 2026 Awards.

Journal of biomolecular techniques : JBT·2026

Same journal

Multisite Assessment of Methods for Cell Preservation Upstream of Single-Cell RNA Sequencing.

Journal of biomolecular techniques : JBT·2026

Same journal

ABRF 2026 Meeting Report.

Journal of biomolecular techniques : JBT·2026

Same journal

Boosting Biomedical Research Capacity: The Role of INBRE-Supported Core Facility Vouchers in IDeA States.

Journal of biomolecular techniques : JBT·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Sep 4, 2025

Setting a Successful Sorting for Extracellular Vesicle Isolation

Setting a Successful Sorting for Extracellular Vesicle Isolation

Published on: October 11, 2024

Cell Sorter Cleaning Practices and Their Impact on Instrument Sterility.

Andrew Box¹, Laura Holmes¹, Monica DeLay²

¹Stowers Institute for Medical Research Kansas City, Missouri 64110 USA.

Journal of Biomolecular Techniques : JBT

|July 15, 2022

Summary

This summary is machine-generated.

Contaminants in cell sorter fluidics can affect experimental results. This study found many cell sorters have endotoxin contamination, and hydrogen peroxide cleaning offers only short-term effectiveness.

Keywords:

RNase contamination endotoxin sorter sterility

More Related Videos

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting FACS

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting FACS

Published on: July 10, 2010

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

Published on: August 7, 2021

Related Experiment Videos

Last Updated: Sep 4, 2025

Setting a Successful Sorting for Extracellular Vesicle Isolation

Setting a Successful Sorting for Extracellular Vesicle Isolation

Published on: October 11, 2024

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting FACS

Purification of Specific Cell Population by Fluorescence Activated Cell Sorting FACS

Published on: July 10, 2010

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

Published on: August 7, 2021

Area of Science:

Biomolecular analysis
Cell biology
Flow cytometry

Background:

Cell sorting is crucial for in vitro and in vivo studies.
Pre- and post-sort processing can impact experimental outcomes.
Previous research indicated site-to-site variability in cell sorting due to environmental factors.

Purpose of the Study:

To assess cell sorter maintenance and contamination levels (bacteria, endotoxin, RNases).
To evaluate the effectiveness of an endotoxin decontamination method.

Main Methods:

Survey of cell sorter maintenance practices in shared resource laboratories.
Testing for bacterial, endotoxin, and RNase contamination.
Evaluation of a hydrogen peroxide-based cleaning procedure for endotoxin removal.

Main Results:

Most cell sorters were free of RNase activity and bacteria.
A significant number of cell sorters exhibited endotoxin contamination.
Hydrogen peroxide cleaning provided only temporary reduction in endotoxin levels.

Conclusions:

Endotoxin contamination is a prevalent issue in cell sorter fluidics.
Current cleaning methods may not offer a lasting solution for endotoxin removal.
Further research is needed to develop effective and sustainable decontamination strategies for cell sorters.