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Updated: Sep 4, 2025

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Molecular characterization of Cordyline virus 1 isolates infecting yam (Dioscorea spp)
Mame Boucar Diouf1,2,3, Olyvia Gaspard1, Armelle Marais4
1INRAE, UR ASTRO, 97170, Petit‑Bourg, Guadeloupe, France.
Abstract:
Cordyline virus 1 (CoV1) is a velarivirus that has so far only been reported in ornamental Ti plants (Cordyline fruticosa). Using high-throughput sequencing, we identified CoV1 infection in yam accessions from Vanuatu. Using a specific RT-PCR assay, we found that CoV1 is also present and highly prevalent in Dioscorea alata, D. cayenensis, and D. trifida in Guadeloupe. Phylogenetic analysis showed that CoV1 isolates infecting yam in Guadeloupe display a low level of molecular diversity. These data provide insights into the transmission of CoV1 in yam in Guadeloupe.
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