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Spatial barcoding-enabled highly multiplexed immunoassay with digital microfluidics.

Huibing Li1, Xianming Liu2, Fengjiao Zhu2

  • 1Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, No.457, Zhongshan Road, Shahekou District, Dalian, Liaoning, 116023, China; College of Stomatology, Dalian Medical University, No. 9, West Section of Lvshun South Road, Lvshunkou District, Dalian, Liaoning, 116044, China.

Biosensors & Bioelectronics
|July 17, 2022
PubMed
Summary
This summary is machine-generated.

This study introduces a novel digital microfluidics platform for highly multiplexed immunoassays. The spatial barcoding approach enables simultaneous detection of over 10 biomarkers from small sample volumes.

Keywords:
AutomationDigital microfluidicsMultiplexed immunoassayProtein detectionSecretion dynamics

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Microfluidics

Background:

  • Digital microfluidics (DMF) offers precise control over microliter samples for immunoassays.
  • Current DMF platforms have limited multiplexing capabilities.
  • There is a need for high-throughput, low-volume immunoassay platforms.

Purpose of the Study:

  • To develop a highly multiplexed digital microfluidics immunoassay platform.
  • To implement a spatial barcoding strategy for enhanced multiplexity.
  • To demonstrate the platform's utility in analyzing biological samples.

Main Methods:

  • A digital microfluidics device was fabricated using a Teflon-coated indium tin oxide (ITO) glass slide for spatial antibody immobilization.
  • Droplets containing analytes and detection reagents were electrically manipulated across electrodes.
  • Immuno-reactions were performed sequentially in parallel for multiplexed detection.

Main Results:

  • The platform achieved high multiplexing (10+ targets) with minimal sample volume (∼4 μL).
  • Excellent sensitivity and reproducibility were demonstrated using recombinant proteins.
  • Temporal profiling of pro-inflammatory cytokine secretion from stimulated macrophages was successfully obtained.

Conclusions:

  • The developed DMF platform with spatial barcoding significantly enhances multiplexity for immunoassays.
  • This technology enables comprehensive protein analysis from biological samples with low volumes.
  • The platform opens new avenues for multiplexed applications in biological research and diagnostics.