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Enzyme-assisted metal nanoparticles etching based plasmonic ELISA: Progress and insights
Maan Mahender Singh1, Jitendra Satija1
1Centre for Nanobiotechnology, VIT, Vellore, Tamil Nadu, 632014, India.
Analytical Biochemistry
|July 19, 2022
Summary
Noble metal nanoparticle etching enhances enzyme-linked immunosorbent assay (ELISA) sensitivity for visual detection. This plasmonic nanoparticle etching approach offers cost-effective, field-deployable biosensing without optical devices.
Area of Science:
- Nanotechnology
- Biosensing
- Analytical Chemistry
Background:
- Noble metal nanoparticles exhibit tunable localized surface plasmon resonance (LSPR) for optical biosensors.
- Existing methods include metal film deposition, nanoparticle aggregation, and synthesis & growth.
- Nanoparticle etching offers superior sensitivity, selectivity, and stability for field applications.
Purpose of the Study:
- To review the state-of-the-art in plasmonic nanoparticle etching-based enzyme-linked immunosorbent assay (ELISA).
- To highlight visual detection capabilities for various analytes.
- To discuss the potential for point-of-care (POC) nanodiagnostics.
Main Methods:
- Review of current literature on nanoparticle etching-based ELISA.
- Discussion of biocatalytic etching mechanisms of plasmonic nanoparticles.
- Analysis of visual color change as an indicator of analyte concentration.
Main Results:
- Plasmonic nanoparticle etching-based ELISA enables sensitive and selective analyte detection.
- Visual detection is achievable without optical readout devices, reducing costs.
- Biocatalytic etching mechanisms lead to distinct color changes correlated with analyte levels.
Conclusions:
- Nanoparticle etching-based ELISA is a promising technique for cost-effective, field-deployable biosensing.
- The method offers advantages in sensitivity, selectivity, and stability.
- Integration with microfluidics can facilitate translation to point-of-care (POC) nanodiagnostics.
