Related Experiment Videos
Experimental hypersensitivity pneumonitis: transfer with cultured cells
The Journal of Laboratory and Clinical Medicine
|June 1, 1987
Summary
Hypersensitivity pneumonitis (HP) can be experimentally transferred using cultured cells from sensitized animals, indicating it is a cell-mediated immune response. Antibody and non-cultured cells did not transfer susceptibility in this guinea pig model.
Area of Science:
- Immunology
- Pulmonary Medicine
- Cellular Biology
Background:
- The roles of antibodies and sensitized cells in hypersensitivity pneumonitis (HP) remain unclear.
- Developing a reliable animal model is crucial for investigating HP mechanisms.
Purpose of the Study:
- To establish an experimental model for hypersensitivity pneumonitis (HP) in guinea pigs.
- To determine if hypersensitivity pneumonitis is transferable via sensitized cells or antibodies.
Main Methods:
- Sensitized guinea pig cells (lymph node, spleen, peritoneal exudate) or serum were transferred to naive recipients.
- Cells were cultured with Micropolyspora faeni extract or concanavalin A (Con A) prior to transfer.
- Recipients were challenged intratracheally with M. faeni and lung abnormalities were assessed.
Main Results:
- Transfer of cultured lymph node or spleen cells (with M. faeni) and cultured peritoneal exudate cells (with Con A) induced significant pulmonary abnormalities.
- Non-cultured cells and antibody-containing serum did not transfer susceptibility to HP.
- Purified protein derivative (PPD) skin reactivity was observed only in recipients of non-cultured cells.
Conclusions:
- Experimental hypersensitivity pneumonitis (HP) can be successfully transferred using cultured cells from sensitized animals.
- The findings strongly suggest that HP is primarily a cell-mediated immune process.
- Antibodies and non-cultured cells do not appear to play a significant role in the transfer of HP susceptibility.