Ecological Traits of Fish for Mercury Biomonitoring: Insights from Compound-Specific Nitrogen and Stable Mercury...

We coupled compound-specific isotopic analyses of nitrogen (N) in amino acids (δ¹⁵N_Glu, δ¹⁵N_Phe) and mercury stable isotopes (δ²⁰²Hg, Δ¹⁹⁹Hg) to quantify ecological traits governing the concentration, variability, and source of Hg in largemouth bass (LB) and pike gudgeon (PG) across four rivers, South Korea. PG displayed uniform Hg concentration (56-137 ng/g), trophic position (TP_corrected; 2.6-3.0, n = 9), and N isotopes in the source amino acid (δ¹⁵N_Phe; 7-13‰), consistent with their specialist feeding on benthic insects. LB showed wide ranges in Hg concentration (45-693 ng/g), TP_corrected (2.8-3.8, n = 14), and δ¹⁵N_Phe (1.3-16‰), reflecting their opportunistic feeding behavior. Hg sources assessed using Hg isotopes reveal low and uniform Δ¹⁹⁹Hg in PG (0.20-0.49‰), similar to Δ¹⁹⁹Hg reported in sediments. LB displayed site-specific δ²⁰²Hg (-0.61 to -0.04‰) and Δ¹⁹⁹Hg (0.53-1.09‰). At the Yeongsan River, LB displayed elevated Δ¹⁹⁹Hg and low δ¹⁵N_Phe, consistent with Hg and N sourced from the atmosphere. LB at the Geum River displayed low Δ¹⁹⁹Hg and high δ¹⁵N_Phe, both similar to the isotope values of anthropogenic sources. Our results suggest that a specialist fish (PG) with consistent ecological traits and Hg concentration is an effective bioindicator species for Hg. When accounting for Hg sources, however, LB better captures site-specific Hg sources.