Biological selenate and selenite reduction by waste activated sludge using hydrogen as electron donor

Biological reduction of selenium oxyanions is widely used for selenium removal from wastewater. The process is, however, limited by the availability of a suitable, efficient and low cost electron donor. In this study, selenite and selenate reduction by waste activated sludge using hydrogen as the electron donor was investigated. Both selenite and selenate (80 mg/L) were completely removed using H₂ within 8 days of incubation. In the presence of sulfate in the medium, the Se removal efficiency decreased to 77.8-95.4% (for selenite) and 88.2-99.4% (for selenate) at different temperatures and initial sulfate concentrations. Thermophilic conditions (50 °C) were better suited for both selenite and selenate reduction using H₂ as electron donor with a 0.8-13.5% increase in overall Se removal. Similarly, sulfate reduction also increased from 69.1- 88% at 30 °C to 72-94.6% at 50 °C. Most of the H₂ utilized was diverted towards Se and sulfate reduction with minimal production of byproducts such as methane (<0.32 mM) or volatile fatty acids (<0.92 mg/L). The elemental Se produced from selenite and selenate reduction ranged between 33.9 and 52.1 mg/L. The elemental selenium nanoparticles produced as a result of selenite and selenate reduction were characterized using transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDX) and dynamic light scattering (DLS) spectroscopy. Furthermore, characterization of the biomass using Fourier-transform infrared spectroscopy (FTIR) and excitation emission matrix (EEM) spectra of the extracellular polymeric substances (EPS) produced by the waste activated sludge were performed to elucidate the mechanism of selenium oxyanion reduction to elemental selenium nanoparticles.