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Involvement of membrane palmitoylated protein 2 (MPP2) in the synaptic molecular complex at the mouse cerebellar

Tomoki Yamada1, Yurika Saitoh1,2, Kiyokazu Kametani1

  • 1Health Science Division, Department of Medical Sciences, Shinshu University Graduate School of Medicine, Science and Technology, 3-1-1 Asahi, Matsumoto, Nagano, 390-8621, Japan.

Histochemistry and Cell Biology
|July 19, 2022
PubMed

Insights

Membrane palmitoylated protein 2 (MPP2) localizes to cerebellar glomeruli postsynaptic regions, interacting with key synaptic proteins. MPP2 influences the distribution of Lin7, crucial for synaptic function in mouse cerebellum.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Molecular Biology

Background:

  • Protein 4.1G interacts with MPP6, Lin7, and CADM4 in peripheral nervous system Schwann cells.
  • MPP6 is a membrane skeletal protein involved in myelin formation.
  • MPP2 is a highly homologous protein to MPP6, necessitating investigation in the central nervous system.

Purpose of the Study:

  • To investigate the localization and protein interactions of MPP2 in the mouse cerebellum.
  • To understand MPP2's role in the postsynaptic region of cerebellar granule cells.
  • To elucidate MPP2's influence on synaptic protein distribution, particularly Lin7.

Main Methods:

  • Immunostaining for MPP2 in mouse cerebellum.
  • High-resolution confocal laser-scanning microscopy (Airyscan mode).
  • Immunoprecipitation analysis to identify protein complexes.
  • Comparison of wild-type and MPP2-deficient mouse cerebella.

Main Results:

  • MPP2 was detected in cerebellar glomeruli (CG) postsynaptic regions and adherens junctions.
  • MPP2 colocalized with CADM1, Lin7, CASK, GluN1, and M-cadherin.
  • MPP2 formed a molecular complex with CADM1, CASK, M-cadherin, and Lin7.
  • MPP2 deficiency altered Lin7 distribution within CG, indicating its role in synaptic organization.

Conclusions:

  • MPP2 is localized to the excitatory postsynaptic region and adherens junctions in cerebellar granule cells.
  • MPP2 forms a complex with several key synaptic proteins, including CADM1, CASK, M-cadherin, and Lin7.
  • MPP2 plays a significant role in regulating the synaptic distribution of Lin7 at postsynaptic sites in the cerebellum.
Keywords:
Cerebellar glomerulusCerebellumLin7MPP2Membrane skeletal protein

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